443143416pSFV1
- 產(chǎn)品貨號GS-1049
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 載體大小11033 bp
The pSFV1 Eukaryotic Expression Vector is a novel DNA expression system based on the Semliki Forest Virus (SFV) replicon which has a broad host range and a high level of expression efficiency (1). In contrast to other protein expression systems, the SFV expression system has several advantages, including direct cloning into the vector and proper glycosylation of the recombinant protein. The SFV expression system is easy to use. The DNA of interest is cloned into the pSFV1 plasmid vector that serves as a template for in vitro synthesis of recombinant RNA, and the RNA is then transfected into tissue culture cells with DMRIE-C Reagent, Lipofectin? Reagent or by electroporation. The recombinant RNA in the cell drives its own replication and capping, resulting in production of large amounts of heterologous protein while inhibiting host protein synthesis. High transfection efficiencies and expression of heterologous proteins have been achieved by transfection of pSFV1 in vitro transcribed RNA into CHO-K1 and BHK-21 cells (2). The plasmid pSFV3- lacZ contains the E. coli #-galactosidase gene, lacZ, and is included as a control plasmid to optimize transfection conditions.
0001 GATGGCGGAT GTGTGACATA CACGACGCCA AAAGATTTTG TTCCAGCTCC TGCCACCTCC
0061 GCTACGCGAG AGATTAACCA CCCACGATGG CCGCCAAAGT GCATGTTGAT ATTGAGGCTG
0121 ACAGCCCATT CATCAAGTCT TTGCAGAAGG CATTTCCGTC GTTCGAGGTG GAGTCATTGC
0181 AGGTCACACC AAATGACCAT GCAAATGCCA GAGCATTTTC GCACCTGGCT ACCAAATTGA
0241 TCGAGCAGGA GACTGACAAA GACACACTCA TCTTGGATAT CGGCAGTGCG CCTTCCAGGA
0301 GAATGATGTC TACGCACAAA TACCACTGCG TATGCCCTAT GCGCAGCGCA GAAGACCCCG
0361 AAAGGCTCGA TAGCTACGCA AAGAAACTGG CAGCGGCCTC CGGGAAGGTG CTGGATAGAG
0421 AGATCGCAGG AAAAATCACC GACCTGCAGA CCGTCATGGC TACGCCAGAC GCTGAATCTC
0481 CTACCTTTTG CCTGCATACA GACGTCACGT GTCGTACGGC AGCCGAAGTG GCCGTATACC
0541 AGGACGTGTA TGCTGTACAT GCACCAACAT CGCTGTACCA TCAGGCGATG AAAGGTGTCA
0601 GAACGGCGTA TTGGATTGGG TTTGACACCA CCCCGTTTAT GTTTGACGCG CTAGCAGGCG
0661 CGTATCCAAC CTACGCCACA AACTGGGCCG ACGAGCAGGT GTTACAGGCC AGGAACATAG
0721 GACTGTGTGC AGCATCCTTG ACTGAGGGAA GACTCGGCAA ACTGTCCATT CTCCGCAAGA
0781 AGCAATTGAA ACCTTGCGAC ACAGTCATGT TCTCGGTAGG ATCTACATTG TACACTGAGA
0841 GCAGAAAGCT ACTGAGGAGC TGGCACTTAC CCTCCGTATT CCACCTGAAA GGTAAACAAT
0901 CCTTTACCTG TAGGTGCGAT ACCATCGTAT CATGTGAAGG GTACGTAGTT AAGAAAATCA
0961 CTATGTGCCC CGGCCTGTAC GGTAAAACGG TAGGGTACGC CGTGACGTAT CACGCGGAGG
1021 GATTCCTAGT GTGCAAGACC ACAGACACTG TCAAAGGAGA AAGAGTCTCA TTCCCTGTAT
1081 GCACCTACGT CCCCTCAACC ATCTGTGATC AAATGACTGG CATACTAGCG ACCGACGTCA
1141 CACCGGAGGA CGCACAGAAG TTGTTAGTGG GATTGAATCA GAGGATAGTT GTGAACGGAA
1201 GAACACAGCG AAACACTAAC ACGATGAAGA ACTATCTGCT TCCGATTGTG GCCGTCGCAT
1261 TTAGCAAGTG GGCGAGGGAA TACAAGGCAG ACCTTGATGA TGAAAAACCT CTGGGTGTCC
1321 GAGAGAGGTC ACTTACTTGC TGCTGCTTGT GGGCATTTAA AACGAGGAAG ATGCACACCA
1381 TGTACAAGAA ACCAGACACC CAGACAATAG TGAAGGTGCC TTCAGAGTTT AACTCGTTCG
1441 TCATCCCGAG CCTATGGTCT ACAGGCCTCG CAATCCCAGT CAGATCACGC ATTAAGATGC
1501 TTTTGGCCAA GAAGACCAAG CGAGAGTTAA TACCTGTTCT CGACGCGTCG TCAGCCAGGG
1561 ATGCTGAACA AGAGGAGAAG GAGAGGTTGG AGGCCGAGCT GACTAGAGAA GCCTTACCAC
1621 CCCTCGTCCC CATCGCGCCG GCGGAGACGG GAGTCGTCGA CGTCGACGTT GAAGAACTAG
1681 AGTATCACGC AGGTGCAGGG GTCGTGGAAA CACCTCGCAG CGCGTTGAAA GTCACCGCAC
1741 AGCCGAACGA CGTACTACTA GGAAATTACG TAGTTCTGTC CCCGCAGACC GTGCTCAAGA
1801 GCTCCAAGTT GGCCCCCGTG CACCCTCTAG CAGAGCAGGT GAAAATAATA ACACATAACG
1861 GGAGGGCCGG CGGTTACCAG GTCGACGGAT ATGACGGCAG GGTCCTACTA CCATGTGGAT
1921 CGGCCATTCC GGTCCCTGAG TTTCAAGCTT TGAGCGAGAG CGCCACTATG GTGTACAACG
1981 AAAGGGAGTT CGTCAACAGG AAACTATACC ATATTGCCGT TCACGGACCG TCGCTGAACA
2041 CCGACGAGGA GAACTACGAG AAAGTCAGAG CTGAAAGAAC TGACGCCGAG TACGTGTTCG
2101 ACGTAGATAA AAAATGCTGC GTCAAGAGAG AGGAAGCGTC GGGTTTGGTG TTGGTGGGAG
2161 AGCTAACCAA CCCCCCGTTC CATGAATTCG CCTACGAAGG GCTGAAGATC AGGCCGTCGG
2221 CACCATATAA GACTACAGTA GTAGGAGTCT TTGGGGTTCC GGGATCAGGC AAGTCTGCTA
2281 TTATTAAGAG CCTCGTGACC AAACACGATC TGGTCACCAG CGGCAAGAAG GAGAACTGCC
2341 AGGAAATAGT TAACGACGTG AAGAAGCACC GCGGGAAGGG GACAAGTAGG GAAAACAGTG
2401 ACTCCATCCT GCTAAACGGG TGTCGTCGTG CCGTGGACAT CCTATATGTG GACGAGGCTT
2461 TCGCTTGCCA TTCCGGTACT CTGCTGGCCC TAATTGCTCT TGTTAAACCT CGGAGCAAAG
2521 TGGTGTTATG CGGAGACCCC AAGCAATGCG GATTCTTCAA TATGATGCAG CTTAAGGTGA
2581 ACTTCAACCA CAACATCTGC ACTGAAGTAT GTCATAAAAG TATATCCAGA CGTTGCACGC
2641 GTCCAGTCAC GGCCATCGTG TCTACGTTGC ACTACGGAGG CAAGATGCGC ACGACCAACC
2701 CGTGCAACAA ACCCATAATC ATAGACACCA CAGGACAGAC CAAGCCCAAG CCAGGAGACA
2761 TCGTGTTAAC ATGCTTCCGA GGCTGGGCAA AGCAGCTGCA GTTGGACTAC CGTGGACACG
2821 AAGTCATGAC AGCAGCAGCA TCTCAGGGCC TCACCCGCAA AGGGGTATAC GCCGTAAGGC
2881 AGAAGGTGAA TGAAAATCCC TTGTATGCCC CTGCGTCGGA GCACGTGAAT GTACTGCTGA
2941 CGCGCACTGA GGATAGGCTG GTGTGGAAAA CGCTGGCCGG CGATCCCTGG ATTAAGGTCC
3001 TATCAAACAT TCCACAGGGT AACTTTACGG CCACATTGGA AGAATGGCAA GAAGAACACG
3061 ACAAAATAAT GAAGGTGATT GAAGGACCGG CTGCGCCTGT GGACGCGTTC CAGAACAAAG
3121 CGAACGTGTG TTGGGCGAAA AGCCTGGTGC CTGTCCTGGA CACTGCCGGA ATCAGATTGA
3181 CAGCAGAGGA GTGGAGCACC ATAATTACAG CATTTAAGGA GGACAGAGCT TACTCTCCAG
3241 TGGTGGCCTT GAATGAAATT TGCACCAAGT ACTATGGAGT TGACCTGGAC AGTGGCCTGT
3301 TTTCTGCCCC GAAGGTGTCC CTGTATTACG AGAACAACCA CTGGGATAAC AGACCTGGTG
3361 GAAGGATGTA TGGATTCAAT GCCGCAACAG CTGCCAGGCT GGAAGCTAGA CATACCTTCC
3421 TGAAGGGGCA GTGGCATACG GGCAAGCAGG CAGTTATCGC AGAAAGAAAA ATCCAACCGC
3481 TTTCTGTGCT GGACAATGTA ATTCCTATCA ACCGCAGGCT GCCGCACGCC CTGGTGGCTG
3541 AGTACAAGAC GGTTAAAGGC AGTAGGGTTG AGTGGCTGGT CAATAAAGTA AGAGGGTACC
3601 ACGTCCTGCT GGTGAGTGAG TACAACCTGG CTTTGCCTCG ACGCAGGGTC ACTTGGTTGT
3661 CACCGCTGAA TGTCACAGGC GCCGATAGGT GCTACGACCT AAGTTTAGGA CTGCCGGCTG
3721 ACGCCGGCAG GTTCGACTTG GTCTTTGTGA ACATTCACAC GGAATTCAGA ATCCACCACT
3781 ACCAGCAGTG TGTCGACCAC GCCATGAAGC TGCAGATGCT TGGGGGAGAT GCGCTACGAC
3841 TGCTAAAACC CGGCGGCATC TTGATGAGAG CTTACGGATA CGCCGATAAA ATCAGCGAAG
3901 CCGTTGTTTC CTCCTTAAGC AGAAAGTTCT CGTCTGCAAG AGTGTTGCGC CCGGATTGTG
3961 TCACCAGCAA TACAGAAGTG TTCTTGCTGT TCTCCAACTT TGACAACGGA AAGAGACCCT
4021 CTACGCTACA CCAGATGAAT ACCAAGCTGA GTGCCGTGTA TGCCGGAGAA GCCATGCACA
4081 CGGCCGGGTG TGCACCATCC TACAGAGTTA AGAGAGCAGA CATAGCCACG TGCACAGAAG
4141 CGGCTGTGGT TAACGCAGCT AACGCCCGTG GAACTGTAGG GGATGGCGTA TGCAGGGCCG
4201 TGGCGAAGAA ATGGCCGTCA GCCTTTAAGG GAGCAGCAAC ACCAGTGGGC ACAATTAAAA
4261 CAGTCATGTG CGGCTCGTAC CCCGTCATCC ACGCTGTAGC GCCTAATTTC TCTGCCACGA
4321 CTGAAGCGGA AGGGGACCGC GAATTGGCCG CTGTCTACCG GGCAGTGGCC GCCGAAGTAA
4381 ACAGACTGTC ACTGAGCAGC GTAGCCATCC CGCTGCTGTC CACAGGAGTG TTCAGCGGCG
4441 GAAGAGATAG GCTGCAGCAA TCCCTCAACC ATCTATTCAC AGCAATGGAC GCCACGGACG
4501 CTGACGTGAC CATCTACTGC AGAGACAAAA GTTGGGAGAA GAAAATCCAG GAAGCCATTG
4561 ACATGAGGAC GGCTGTGGAG TTGCTCAATG ATGACGTGGA GCTGACCACA GACTTGGTGA
4621 GAGTGCACCC GGACAGCAGC CTGGTGGGTC GTAAGGGCTA CAGTACCACT GACGGGTCGC
4681 TGTACTCGTA CTTTGAAGGT ACGAAATTCA ACCAGGCTGC TATTGATATG GCAGAGATAC
4741 TGACGTTGTG GCCCAGACTG CAAGAGGCAA ACGAACAGAT ATGCCTATAC GCGCTGGGCG
4801 AAACAATGGA CAACATCAGA TCCAAATGTC CGGTGAACGA TTCCGATTCA TCAACACCTC
4861 CCAGGACAGT GCCCTGCCTG TGCCGCTACG CAATGACAGC AGAACGGATC GCCCGCCTTA
4921 GGTCACACCA AGTTAAAAGC ATGGTGGTTT GCTCATCTTT TCCCCTCCCG AAATACCATG
4981 TAGATGGGGT GCAGAAGGTA AAGTGCGAGA AGGTTCTCCT GTTCGACCCG ACGGTACCTT
5041 CAGTGGTTAG TCCGCGGAAG TATGCCGCAT CTACGACGGA CCACTCAGAT CGGTCGTTAC
5101 GAGGGTTTGA CTTGGACTGG ACCACCGACT CGTCTTCCAC TGCCAGCGAT ACCATGTCGC
5161 TACCCAGTTT GCAGTCGTGT GACATCGACT CGATCTACGA GCCAATGGCT CCCATAGTAG
5221 TGACGGCTGA CGTACACCCT GAACCCGCAG GCATCGCGGA CCTGGCGGCA GATGTGCACC
5281 CTGAACCCGC AGACCATGTG GACCTCGAGA ACCCGATTCC TCCACCGCGC CCGAAGAGAG
5341 CTGCATACCT TGCCTCCCGC GCGGCGGAGC GACCGGTGCC GGCGCCGAGA AAGCCGACGC
5401 CTGCCCCAAG GACTGCGTTT AGGAACAAGC TGCCTTTGAC GTTCGGCGAC TTTGACGAGC
5461 ACGAGGTCGA TGCGTTGGCC TCCGGGATTA CTTTCGGAGA CTTCGACGAC GTCCTGCGAC
5521 TAGGCCGCGC GGGTGCATAT ATTTTCTCCT CGGACACTGG CAGCGGACAT TTACAACAAA
5581 AATCCGTTAG GCAGCACAAT CTCCAGTGCG CACAACTGGA TGCGGTCCAG GAGGAGAAAA
5641 TGTACCCGCC AAAATTGGAT ACTGAGAGGG AGAAGCTGTT GCTGCTGAAA ATGCAGATGC
5701 ACCCATCGGA GGCTAATAAG AGTCGATACC AGTCTCGCAA AGTGGAGAAC ATGAAAGCCA
5761 CGGTGGTGGA CAGGCTCACA TCGGGGGCCA GATTGTACAC GGGAGCGGAC GTAGGCCGCA
5821 TACCAACATA CGCGGTTCGG TACCCCCGCC CCGTGTACTC CCCTACCGTG ATCGAAAGAT
5881 TCTCAAGCCC CGATGTAGCA ATCGCAGCGT GCAACGAATA CCTATCCAGA AATTACCCAA
5941 CAGTGGCGTC GTACCAGATA ACAGATGAAT ACGACGCATA CTTGGACATG GTTGACGGGT
6001 CGGATAGTTG CTTGGACAGA GCGACATTCT GCCCGGCGAA GCTCCGGTGC TACCCGAAAC
6061 ATCATGCGTA CCACCAGCCG ACTGTACGCA GTGCCGTCCC GTCACCCTTT CAGAACACAC
6121 TACAGAACGT GCTAGCGGCC GCCACCAAGA GAAACTGCAA CGTCACGCAA ATGCGAGAAC
6181 TACCCACCAT GGACTCGGCA GTGTTCAACG TGGAGTGCTT CAAGCGCTAT GCCTGCTCCG
6241 GAGAATATTG GGAAGAATAT GCTAAACAAC CTATCCGGAT AACCACTGAG AACATCACTA
6301 CCTATGTGAC CAAATTGAAA GGCCCGAAAG CTGCTGCCTT GTTCGCTAAG ACCCACAACT
6361 TGGTTCCGCT GCAGGAGGTT CCCATGGACA GATTCACGGT CGACATGAAA CGAGATGTCA
6421 AAGTCACTCC AGGGACGAAA CACACAGAGG AAAGACCCAA AGTCCAGGTA ATTCAAGCAG
6481 CGGAGCCATT GGCGACCGCT TACCTGTGCG GCATCCACAG GGAATTAGTA AGGAGACTAA
6541 ATGCTGTGTT ACGCCCTAAC GTGCACACAT TGTTTGATAT GTCGGCCGAA GACTTTGACG
6601 CGATCATCGC CTCTCACTTC CACCCAGGAG ACCCGGTTCT AGAGACGGAC ATTGCATCAT
6661 TCGACAAAAG CCAGGACGAC TCCTTGGCTC TTACAGGTTT AATGATCCTC GAAGATCTAG
6721 GGGTGGATCA GTACCTGCTG GACTTGATCG AGGCAGCCTT TGGGGAAATA TCCAGCTGTC
6781 ACCTACCAAC TGGCACGCGC TTCAAGTTCG GAGCTATGAT GAAATCGGGC ATGTTTCTGA
6841 CTTTGTTTAT TAACACTGTT TTGAACATCA CCATAGCAAG CAGGGTACTG GAGCAGAGAC
6901 TCACTGACTC CGCCTGTGCG GCCTTCATCG GCGACGACAA CATCGTTCAC GGAGTGATCT
6961 CCGACAAGCT GATGGCGGAG AGGTGCGCGT CGTGGGTCAA CATGGAGGTG AAGATCATTG
7021 ACGCTGTCAT GGGCGAAAAA CCCCCATATT TTTGTGGGGG ATTCATAGTT TTTGACAGCG
7081 TCACACAGAC CGCCTGCCGT GTTTCAGACC CACTTAAGCG CCTGTTCAAG TTGGGTAAGC
7141 CGCTAACAGC TGAAGACAAG CAGGACGAAG ACAGGCGACG AGCACTGAGT GACGAGGTTA
7201 GCAAGTGGTT CCGGACAGGC TTGGGGGCCG AACTGGAGGT GGCACTAACA TCTAGGTATG
7261 AGGTAGAGGG CTGCAAAAGT ATCCTCATAG CCATGGCCAC CTTGGCGAGG GACATTAAGG
7321 CGTTTAAGAA ATTGAGAGGA CCTGTTATAC ACCTCTACGG CGGTCCTAGA TTGGTGCGTT
7381 AATACACAGA ATTCTGATTG GATCCCGGGT AATTAATTGA ATTACATCCC TACGCAAACG
7441 TTTTACGGCC GCCGGTGGCG CCCGCGCCCG GCGGCCCGTC CTTGGCCGTT GCAGGCCACT
7501 CCGGTGGCTC CCGTCGTCCC CGACTTCCAG GCCCAGCAGA TGCAGCAACT CATCAGCGCC
7561 GTAAATGCGC TGACAATGAG ACAGAACGCA ATTGCTCCTG CTAGGCCTCC CAAACCAAAG
7621 AAGAAGAAGA CAACCAAACC AAAGCCGAAA ACGCAGCCCA AGAAGATCAA CGGAAAAACG
7681 CAGCAGCAAA AGAAGAAAGA CAAGCAAGCC GACAAGAAGA AGAAGAAACC CGGAAAAAGA
7741 GAAAGAATGT GCATGAAGAT TGAAAATGAC TGTATCTTCG TATGCGGCTA GCCACAGTAA
7801 CGTAGTGTTT CCAGACATGT CGGGCACCGC ACTATCATGG GTGCAGAAAA TCTCGGGTGG
7861 TCTGGGGGCC TTCGCAATCG GCGCTATCCT GGTGCTGGTT GTGGTCACTT GCATTGGGCT
7921 CCGCAGATAA GTTAGGGTAG GCAATGGCAT TGATATAGCA AGAAAATTGA AAACAGAAAA
7981 AGTTAGGGTA AGCAATGGCA TATAACCATA ACTGTATAAC TTGTAACAAA GCGCAACAAG
8041 ACCTGCGCAA TTGGCCCCGT GGTCCGCCTC ACGGAAACTC GGGGCAACTC ATATTGACAC
8101 ATTAATTGGC AATAATTGGA AGCTTACATA AGCTTAATTC GACGAATAAT TGGATTTTTA
8161 TTTTATTTTG CAATTGGTTT TTAATATTTC CAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
8221 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA ACTAGTCTGC ATTAATGAAT
8281 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
8341 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
8401 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
8461 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
8521 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
8581 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
8641 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG
8701 CTCGCGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
8761 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
8821 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
8881 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
8941 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
9001 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
9061 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
9121 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
9181 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
9241 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
9301 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
9361 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
9421 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
9481 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
9541 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
9601 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
9661 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
9721 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
9781 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
9841 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
9901 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
9961 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
10021 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
10081 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
10141 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
10201 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA
10261 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG
10321 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT
10381 CACAGCTTCT GTCTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG
10441 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT
10501 GCACCATATC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTACTAGG
10561 TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC
10621 AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG
10681 AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA
10741 CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCTG GCTAGCGATG
10801 ACCCTGCTGA TTGGTTCGCT GACCATTTCC GGGGTGCGGA ACGGCGTTAC CAGAAACTCA
10861 GAAGGTTCGT CCAACCAAAC CGACTCTGAC GGCAGTTTAC GAGAGAGATG ATAGGGTCTG
10921 CTTCAGTAAG CCAGATGCTA CACAATTAGG CTTGTACATA TTGTCGTTAG AACGCGGCTA
10981 CAATTAATAC ATAACCTTAT GTATCATACA CATACGATTT AGGTGACACT ATA