載體說(shuō)明

pOG44 is a 5.8 kb Flp recombinase expression vector designed for use with the Flp-In. System (Catalog nos. K6010-01 and K6010-02) available from Invitrogen. When cotransfected with the pcDNA5/FRT plasmid into a Flp-In. mammalian host cell line, the Flp recombinase expressed from pOG44 mediates integration of the pcDNA5/FRT vector containing the gene of interest into the genome via Flp Recombination Target (FRT) sites. The vector contains the following elements:

• The human cytomegalovirus (CMV) immediate-early enhancer/promoter for highlevel constitutive expression of the Flp recombinase in a wide range of mammalian cells (Andersson et al., 1989; Boshart et al., 1985; Nelson et al., 1987)
• Synthetic intron to enhance expression of the FLP gene (Huang and Gorman, 1990; O’Gorman et al., 1991)
• FLP gene encoding the Flp recombinase (Buchholz et al., 1996) to mediate integration of the pcDNA5/FRT expression plasmid into the genome