443143416pNZ8148
- 產(chǎn)品貨號GS-1094
- 銷售價格￥ 1020
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 載體大小3167 bp
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.
The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus.
pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).
0001 AGATCTAGTC TTATAACTAT ACTGACAATA GAAACATTAA CAAATCTAAA ACAGTCTTAA
0061 TTCTATCTTG AGAAAGTATT GGTAATAATA TTATTGTCGA TAACGCGAGC ATAATAAACG
0121 GCTCTGATTA AATTCTGAAG TTTGTTAGAT ACAATGATTT CGTTCGAAGG AACTACAAAA
0181 TAAATTATAA GGAGGCACTC ACCATGGGTA CTGCAGGCAT GCGGTACCAC TAGTTCTAGA
0241 GAGCTCAAGC TTTCTTTGAA CCAAAATTAG AAAACCAAGG CTTGAAACGT TCAATTGAAA
0301 TGGCAATTAA ACAAATTACA GCACGTGTTG CTTTGATTGA TAGCCAAAAA GCAGCAGTTG
0361 ATAAAGCAAT TACTGATATT GCTGAAAAAT TGTAATTTAT AAATAAAAAT CACCTTTTAG
0421 AGGTGGTTTT TTTATTTATA AATTATTCGT TTGATTTCGC TTTCGATAGA ACAATCAAAT
0481 CGTTTCTGAG ACGTTTTAGC GTTTATTTCG TTTAGTTATC GGCATAATCG TTAAAACAGG
0541 CGTTATCGTA GCGTAAAAGC CCTTGAGCGT AGCGTGGCTT TGCAGCGAAG ATGTTGTCTG
0601 TTAGATTATG AAAGCCGATG ACTGAATGAA ATAATAAGCG CAGCGTCCTT CTATTTCGGT
0661 TGGAGGAGGC TCAAGGGAGT TTGAGGGAAT GAAATTCCCT CATGGGTTTG ATTTTAAAAA
0721 TTGCTTGCAA TTTTGCCGAG CGGTAGCGCT GGAAAATTTT TGAAAAAAAT TTGGAATTTG
0781 GAAAAAAATG GGGGGAAAGG AAGCGAATTT TGCTTCCGTA CTACGACCCC CCATTAAGTG
0841 CCGAGTGCCA ATTTTTGTGC CAAAAACGCT CTATCCCAAC TGGCTCAAGG GTTTGAGGGG
0901 TTTTTCAATC GCCAACGAAT CGCCAACGTT TTCGCCAACG TTTTTTATAA ATCTATATTT
0961 AAGTAGCTTT ATTTTTGTTT TTATGATTAC AAAGTGATAC ACTAATTTTA TAAAATTATT
1021 TGATTGGAGT TTTTTAAATG GTGATTTCAG AATCGAAAAA AAGAGTTATG ATTTCTCTGA
1081 CAAAAGAGCA AGATAAAAAA TTAACAGATA TGGCGAAACA AAAAGATTTT TCAAAATCTG
1141 CGGTTGCGGC GTTAGCTATA GAAGAATATG CAAGAAAGGA ATCAGAACAA AAAAAATAAG
1201 CGAAAGCTCG CGTTTTTAGA AGGATACGAG TTTTCGCTAC TTGTTTTTGA TAAGGTAATT
1261 ATATCATGGC TATTAAAAAT ACTAAAGCTA GAAATTTTGG ATTTTTATTA TATCCTGACT
1321 CAATTCCTAA TGATTGGAAA GAAAAATTAG AGAGTTTGGG CGTATCTATG GCTGTCAGTC
1381 CTTTACACGA TATGGACGAA AAAAAAGATA AAGATACATG GAATAGTAGT GATGTTATAC
1441 GAAATGGAAA GCACTATAAA AAACCACACT ATCACGTTAT ATATATTGCA CGAAATCCTG
1501 TAACAATAGA AAGCGTTAGG AACAAGATTA AGCGAAAATT GGGGAATAGT TCAGTTGCTC
1561 ATGTTGAGAT ACTTGATTAT ATCAAAGGTT CATATGAATA TTTGACTCAT GAATCAAAGG
1621 ACGCTATTGC TAAGAATAAA CATATATACG ACAAAAAAGA TATTTTGAAC ATTAATGATT
1681 TTGATATTGA CCGCTATATA ACACTTGATG AAAGCCAAAA AAGAGAATTG AAGAATTTAC
1741 TTTTAGATAT AGTGGATGAC TATAATTTGG TAAATACAAA AGATTTAATG GCTTTTATTC
1801 GCCTTAGGGG AGCGGAGTTT GGAATTTTAA ATACGAATGA TGTAAAAGAT ATTGTTTCAA
1861 CAAACTCTAG CGCCTTTAGA TTATGGTTTG AGGGCAATTA TCAGTGTGGA TATAGAGCAA
1921 GTTATGCAAA GGTTCTTGAT GCTGAAACGG GGGAAATAAA ATGACAAACA AAGAAAAAGA
1981 GTTATTTGCT GAAAATGAGG AATTAAAAAA AGAAATTAAG GACTTAAAAG AGCGTATTGA
2041 AAGATACAGA GAAATGGAAG TTGAATTAAG TACAACAATA GATTTATTGA GAGGAGGGAT
2101 TATTGAATAA ATAAAAGCCC CCCTGACGAA AGTCGACGGC AATAGTTACC CTTATTATCA
2161 AGATAAGAAA GAAAAGGATT TTTCGCTACG CTCAAATCCT TTAAAAAAAC ACAAAAGACC
2221 ACATTTTTTA ATGTGGTCTT TTATTCTTCA ACTAAAGCAC CCATTAGTTC AACAAACGAA
2281 AATTGGATAA AGTGGGATAT TTTTAAAATA TATATTTATG TTACAGTAAT ATTGACTTTT
2341 AAAAAAGGAT TGATTCTAAT GAAGAAAGCA GACAAGTAAG CCTCCTAAAT TCACTTTAGA
2401 TAAAAATTTA GGAGGCATAT CAAATGAACT TTAATAAAAT TGATTTAGAC AATTGGAAGA
2461 GAAAAGAGAT ATTTAATCAT TATTTGAACC AACAAACGAC TTTTAGTATA ACCACAGAAA
2521 TTGATATTAG TGTTTTATAC CGAAACATAA AACAAGAAGG ATATAAATTT TACCCTGCAT
2581 TTATTTTCTT AGTGACAAGG GTGATAAACT CAAATACAGC TTTTAGAACT GGTTACAATA
2641 GCGACGGAGA GTTAGGTTAT TGGGATAAGT TAGAGCCACT TTATACAATT TTTGATGGTG
2701 TATCTAAAAC ATTCTCTGGT ATTTGGACTC CTGTAAAGAA TGACTTCAAA GAGTTTTATG
2761 ATTTATACCT TTCTGATGTA GAGAAATATA ATGGTTCGGG GAAATTGTTT CCCAAAACAC
2821 CTATACCTGA AAATGCTTTT TCTCTTTCTA TTATTCCTTG GACTTCATTT ACTGGGTTTA
2881 ACTTAAATAT CAATAATAAT AGTAATTACC TTCTACCCAT TATTACAGCA GGAAAATTCA
2941 TTAATAAAGG TAATTCAATA TATTTACCGC TATCTTTACA GGTACATCAT TCTGTTTGTG
3001 ATGGTTATCA TGCTGGATTG TTTATGAACT CTATTCAGGA ATTGTCAGAT AGGCCTAATG
3061 ACTGGCTTTT ATAATATGAG ATAATGCCGA CTGTACTTTT TACAGTCGGT TTTCTAATGT
3121 CACTAACCTG CCCCGTTAGT TGAAGAAGGT TTTTATATTA CAGCTCC