443143416pMIB/v5-His B
- 產(chǎn)品貨號GS-1176
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 表達(dá)類型高
- 載體抗性Ampicillin (氨芐青霉素)
- 篩選標(biāo)記Blasticidin
- 載體大小3600 bp
- 啟動子OpIE2
- 5'測序引物OpIE2-F:?5'd[CGCAACGATCTGGTAAACAC]3'
- 備注說明在昆蟲細(xì)胞中能夠穩(wěn)定表達(dá)，HBM標(biāo)簽可實(shí)現(xiàn)目的蛋白的分泌表達(dá)
載體描述
pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five? insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
- The OpIE2 promoter for constitutive expression
- The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
- The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
- C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
