443143416pACT2 AD
- 產(chǎn)品貨號GS-1646
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 載體抗性Ampicillin (氨芐青霉素)
- 載體大小8117 bp
- 啟動子ADH1
pACT2 generates a fusion of the GAL4 AD (amino acids 768–881), an HA epitope tag, and a protein of interest (or protein encoded by a cDNA in a fusion library) cloned into the MCS in the correct orientation and reading frame. pACT2, which is derived from pACT (1), contains a unique EcoR I site in the MCS. The hybrid protein is expressed at high levels in yeast host cells from the constitutive ADH1 promoter (P); transcription is terminated at the ADH1 transcription termination signal (T). The protein is targeted to the yeast nucleus by the nuclear localization sequence from SV40 T-antigen which has been cloned into the 5’ end of the GAL4 AD sequence (2). pACT2 is a shuttle vector that replicates autonomously in both E. coli and S. cerevisiae and carries the bla gene, which confers ampicillin resistance in E. coli. pACT2 also contains the LEU2 nutritional gene that allows yeast auxotrophs to grow on limiting synthetic media. Transformants with AD/library plasmids can be selected by complementation by the LEU2 gene by using an E. coli strain that carries a leuB mutation (e.g., HB101).
0001 GCTTTGAAGA AAAATGCGCC TTATTCAATC TTTGCTATAA AAAATGGCCC AAAATCTCAC
0061 ATTGGAAGAC ATTTGATGAC CTCATTTCTT TCAATGAAGG GCCTAACGGA GTTGACTAAT
0121 GTTGTGGGAA ATTGGAGCGA TAAGCGTGCT TCTGCCGTGG CCAGGACAAC GTATACTCAT
0181 CAGATAACAG CAATACCTGA TCACTACTTC GCACTAGTTT CTCGGTACTA TGCATATGAT
0241 CCAATATCAA AGGAAATGAT AGCATTGAAG GATGAGACTA ATCCAATTGA GGAGTGGCAG
0301 CATATAGAAC AGCTAAAGGG TAGTGCTGAA GGAAGCATAC GATACCCCGC ATGGAATGGG
0361 ATAATATCAC AGGAGGTACT AGACTACCTT TCATCCTACA TAAATAGACG CATATAAGTA
0421 CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT GTATATATAT ATACAGGCAA
0481 CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT GCGCAGCTCG CGTTGCATTT
0541 TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT TCCGAAGTTC CTATTCTCTA
0601 GAAAGTATAG GAACTTCAGA GCGCTTTTGA AAACCAAAAG CGCTCTGAAG ACGCACTTTC
0661 AAAAAACCAA AAACGCACCG GACTGTAACG AGCTACTAAA ATATTGCGAA TACCGCTTCC
0721 ACAAACATTG CTCAAAAGTA TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC
0781 CTACCCATCC ACCTTTCGCT CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT
0841 GTTTATCTCT AGTATTACTC TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG
0901 AATCGAAAAC AATACGAAAA TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG
0961 AAGAAACCGT TCATAATTTT CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC
1021 ACAAAGTATG CGCAATCCAC ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA
1081 AAATGCACCC GCAGCTTCGC TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT
1141 TATGGAAGAG AAAATAGACA CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC
1201 AAAAAGTTAT CAAGAGACTG CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA
1261 TGCTTTGTTA GAAAAATAGC GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA
1321 GATTCTTTGT TGGTAAAATA GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT
1381 CAGATTCTTT GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTTTA CAAAAATGAA
1441 GCACAGATTC TTCGTTGGTA AAATAGCGCT TTCGCGTTGC ATTTCTGTTC TGTAAAAATG
1501 CAGCTCAGAT TCTTTGTTTG AAAAATTAGC GCTCTCGCGT TGCATTTTTG TTCTACAAAA
1561 TGAAGCACAG ATGCTTCGTT AACAAAGATA TGCTATTGAA GTGCAAGATG GAAACGCAGA
1621 AAATGAACCG GGGATGCGAC GTGCAAGATT ACCTATGCAA TAGATGCAAT AGTTTCTCCA
1681 GGAACCGAAA TACATACATT GTCTTCCGTA AAGCGCTAGA CTATATATTA TTATACAGGT
1741 TCAAATATAC TATCTGTTTC AGGGAAAACT CCCAGGTTCG GATGTTCAAA ATTCAATGAT
1801 GGGTAACAAG TACGATCGTA AATCTGTAAA ACAGTTTGTC GGATATTAGG CTGTATCTCC
1861 TCAAAGCGTA TTCGAATATC ATTGAGAAGC TGCAGCGTCA CATCGGATAA TAATGATGGC
1921 AGCCATTGTA GAAGTGCCTT TTGCATTTCT AGTCTCTTTC TCGGTCTAGC TAGTTTTACT
1981 ACATCGCGAA GATAGAATCT TAGATCACAC TGCCTTTGCT GAGCTGGATC AATAGAGTAA
2041 CAAAAGAGTG GTAAGGAACT GTGGGAATAC TCAGGTATCG TAAGATGCAA GAGTTCGAAT
2101 CTCTTAGCAA CCATTATTTT TTTCCTCAAC ATAACGAGAA CACACAGGGG CGCTATCGCA
2161 CAGAATCAAA TTCGATGACT GGAAATTTTT TGTTAATTTC AGAGGTCGCC TGACGCATAT
2221 ACCTTTTTCA ACTGAAAAAT TGGGAGAAAA AGGAAAGGTG AGAGGCCGGA ACCGGCTTTT
2281 CATATAGAAT AGAGAAGCGT TCATGACTAA ATGCTTGCAT CACAATACTT GAAGTTGACA
2341 ATATTATTTA AGGACCTATT GTTTTTTCCA ATAGGTGGTT AGCAATCGTC TTACTTTCTA
2401 ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA TTTCAAGGAT ATACCATTCT
2461 AATGTCTGCC CCTATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG
2521 TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA
2581 TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT
2641 CCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGTT GATGCCGTTT TGTTAGGTGC
2701 TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT
2761 CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT
2821 TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG
2881 AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC
2941 TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT
3001 CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT
3061 GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AATTTCCTAC
3121 ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA AGAACCCAAC
3181 CCACCTAAAT GGTATTATAA TCACCAGCAA CATGTTTGGT GATATCATCT CCGATGAAGC
3241 CTCCGTTATC CCAGGTTCCT TGGGTTTGTT GCCATCTGCG TCCTTGGCCT CTTTGCCAGA
3301 CAAGAACACC GCATTTGGTT TGTACGAACC ATGCCACGGT TCTGCTCCAG ATTTGCCAAA
3361 GAATAAGGTT GACCCTATCG CCACTATCTT GTCTGCTGCA ATGATGTTGA AATTGTCATT
3421 GAACTTGCCT GAAGAAGGTA AGGCCATTGA AGATGCAGTT AAAAAGGTTT TGGATGCAGG
3481 TATCAGAACT GGTGATTTAG GTGGTTCCAA CAGTACCACC GAAGTCGGTG ATGCTGTCGC
3541 CGAAGAAGTT AAGAAAATCC TTGCTTAAAA AGATTCTCTT TTTTTATGAT ATTTGTACAT
3601 AAACTTTATA AATGAAATTC ATAATAGAAA CGACACGAAA TTACAAAATG GAATATGTTC
3661 ATAGGGTAGA CGAAACTATA TACGCAATCT ACATACATTT ATCAAGAAGG AGAAAAAGGA
3721 GGATAGTAAA GGAATACAGG TAAGCAAATT GATACTAATG GCTCAACGTG ATAAGGAAAA
3781 AGAATTGCAC TTTAACATTA ATATTGACAA GGAGGAGGGC ACCACACAAA AAGTTAGGTG
3841 TAACAGAAAA TCATGAAACT ACGATTCCTA ATTTGATATT GGAGGATTTT CTCTAAAAAA
3901 AAAAAAATAC AACAAATAAA AAACACTCAA TGACCTGACC ATTTGATGGA GTTTAAGTCA
3961 ATACCTTCTT GAACCATTTC CCATAATGGT GAAAGTTCCC TCAAGAATTT TACTCTGTCA
4021 GAAACGGCCT TAACGACGTA GTCGACCAAT TCCGATCATA TCAATAACCC TTAATATAAC
4081 TTCGTATAAT GTATGCTATC GAAGTTATTA GGTCTGAAGA GGAGTTTACG TCCAGCCAAG
4141 CTAGCCTGGC TGCAGGTCGA GCGGCCGCGA TCCGGAACCC TTAATATAAC TTCGTATAAT
4201 GTATGCTATA CGAAGTTATT AGGTCCCTCG ACCAATTCCG ATCATATTCA ATAACCCTTA
4261 ATATAACTTC GTATAATGTA TGCTATACGA AGTTATTAGG TCTGAAGAGG AGTTTACGTC
4321 CGCTAGCTTG GCTGCAGGTC GAGCGGCCGC GATCCGGAAC CCTTAATATA ACTTCGTATA
4381 ATGTATGCTA TACGAAGTTA TTAGGTCCCT CGCACATGCC GGTAGAGGTG TGGTCAATAA
4441 GAGCGACCTC ATGCTATACC TGAGAAAGCA ACCTGACCTA CAGGAAAGAG TTACTCAAGA
4501 ACAAGAATTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA TTTGTATACA CTTATTTTTT
4561 TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT TTAGAAGTGT
4621 CAACAACGTA TCTACCAACG ATTTGACCCT TTTCCATCTT TTCGTAAATT TCTGGCAAGG
4681 TAGACAAGCC GACAACCTTG ATTGGAGACT TGACCAAACC TCTGGCGAAG AAGTCCAAAG
4741 CTTCTGAATA AGCCCTCGTA ATATATTTTC ATGAAGAATT TAGGTCCAAA AAAAAGATGG
4801 GCATTAATTC TAGTCATTTA AAAAATTCTA TAGATCAGAG GTTACATGGC CAAGATTGAA
4861 ACTTAGAGGA GTATAGTTAC ATAAAAGAAG GCAAAACGAT GTATAAATGA AAGAAATTGA
4921 GATGGTGCAC GATGCACAGT TGAAGTGAAC TTGCGGGGTT TTTCAGTATC TACGATTCAT
4981 AGATCTCTCG AGCTCGAATT CGGATCCCCG GGGCCTCCAT GGCCATATGA CCACCCAAGC
5041 TAGCGTAATC TGGAACATCG TATGGGTAAG CCATACAGAT CTCTTTTTTT GGGTTTGGTG
5101 GGGTATCTTC ATCATCGAAT AGATAGTTAT ATACATCATC CATTGTAGTG GTATTAAACA
5161 TCCCTGTAGT GATTCCAAAC GCGTTATACG CAGTTTGGTC CGTCCAACCA GGTGACAGTG
5221 GTTTTGAATT ATTACCATCA TCAATTTTAC TAGCCGTGAT TTCATTATTC ATGAAGTTAT
5281 CATGAACGTT AGAGGAGGCA ATTGGTTGTG AAAGCGCTTG AGAATTTGTT TGAGTTGTTA
5341 TGAGGTTCGG ACCGTTGCTA CTGTTAGTGA AAGTGAAGGA CAATGAGCTA TCAGCAATAT
5401 TCCCACTTTG ATTAAAATTG GCGGCGGTAC CCAATTCGAC CTTTCTCTTC TTTTTTGGAG
5461 GCTCGGGAAT TAATTCCGCT TTATCCATCT TTGCAAAGCT TGGAGTTGAT TGTATGCTTG
5521 GTATAGCTTG AAATATTGTG CAGAAAAAGA AACAAGGAAG AAAGGGAACG AGAACAATGA
5581 CGAGGAAACA AAAGATTAAT AATTGCAGGT CTATTTATAC TTGATAGCAA AGCGGCAAAC
5641 TTTTTTTATT TCAAATTCAA GTAACTGGAA GGAAGGCCGT ATACCGTTGC TCATTAGAGA
5701 GTAGTGTGCG TGAATGAAGG AAGGAAAAAG TTTCGTGTGT TCGAAGATAC CCCTCATCAG
5761 CTCTGGAACA ACGACATCTG TTGGTGCTGT CTTTGTCGTT AATTTTTTCC TTTAGTGTCT
5821 TCCATCATTT TTTTTGTCAT TGCGGATATG GTGAGACAAC AACGGGGGAG AGAGAAAAGA
5881 AAAAAAAAGA AAAGAAGTTG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA
5941 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA
6001 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA
6061 CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA
6121 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA
6181 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
6241 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
6301 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
6361 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
6421 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
6481 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
6541 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
6601 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
6661 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
6721 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
6781 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
6841 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
6901 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
6961 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
7021 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
7081 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
7141 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
7201 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
7261 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
7321 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
7381 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
7441 CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
7501 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
7561 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
7621 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
7681 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
7741 CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
7801 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
7861 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
7921 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
7981 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
8041 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
8101 CCCGAAAAGT GCCACCT