pGL4.26[luc2/minP/Hygro]
- 產(chǎn)品貨號(hào)GS-2048
- 銷售價(jià)格¥ 1020
- 包裝規(guī)格2μg質(zhì)?;?.5ml甘油菌
- 載體抗性Ampicillin (氨芐青霉素)
- 篩選標(biāo)記Hygromycin (潮霉素)
- 載體大小5882 bp
- 備注說明Vector with a minimal promoter for measuring the activity of transcriptional response elements using a luciferase assay.
The pGL4.26[luc2/minP/Hygro] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.
0001 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
0061 GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG ACTTCCAGCT TGGCAATCCG
0121 GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT
0181 TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG
0241 CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG
0301 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA
0361 CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG
0421 GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC
0481 TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC
0541 AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA
0601 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC
0661 CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC
0721 TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA
0781 CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG
0841 ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG
0901 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT
0961 TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT
1021 TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA
1081 GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC
1141 CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG
1201 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG
1261 TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG
1321 GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA
1381 AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA
1441 TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC
1501 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG
1561 ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA
1621 CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG
1681 GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA
1741 AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATAATTCT
1801 AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
1861 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
1921 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
1981 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA
2041 AATGTGGTAA AATCGATAAG GATCCGTTTG CGTATTGGGC GCTCTTCCGC TGATCTGCGC
2101 AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGCTACCT TCTGAGGCGG
2161 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
2221 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC
2281 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT
2341 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC
2401 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT
2461 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCGATTC
2521 TTCTGACACT AGCGCCACCA TGAAGAAGCC CGAACTCACC GCTACCAGCG TTGAAAAATT
2581 TCTCATCGAG AAGTTCGACA GTGTGAGCGA CCTGATGCAG TTGTCGGAGG GCGAAGAGAG
2641 CCGAGCCTTC AGCTTCGATG TCGGCGGACG CGGCTATGTA CTGCGGGTGA ATAGCTGCGC
2701 TGATGGCTTC TACAAAGACC GCTACGTGTA CCGCCACTTC GCCAGCGCTG CACTACCCAT
2761 CCCCGAAGTG TTGGACATCG GCGAGTTCAG CGAGAGCCTG ACATACTGCA TCAGTAGACG
2821 CGCCCAAGGC GTTACTCTCC AAGACCTCCC CGAAACAGAG CTGCCTGCTG TGTTACAGCC
2881 TGTCGCCGAA GCTATGGATG CTATTGCCGC CGCCGACCTC AGTCAAACCA GCGGCTTCGG
2941 CCCATTCGGG CCCCAAGGCA TCGGCCAGTA CACAACCTGG CGGGATTTCA TTTGCGCCAT
3001 TGCTGATCCC CATGTCTACC ACTGGCAGAC CGTGATGGAC GACACCGTGT CCGCCAGCGT
3061 AGCTCAAGCC CTGGACGAAC TGATGCTGTG GGCCGAAGAC TGTCCCGAGG TGCGCCACCT
3121 CGTCCATGCC GACTTCGGCA GCAACAACGT CCTGACCGAC AACGGCCGCA TCACCGCCGT
3181 AATCGACTGG TCCGAAGCTA TGTTCGGGGA CAGTCAGTAC GAGGTGGCCA ACATCTTCTT
3241 CTGGCGGCCC TGGCTGGCTT GCATGGAGCA GCAGACTCGC TACTTCGAGC GCCGGCATCC
3301 CGAGCTGGCC GGCAGCCCTC GTCTGCGAGC CTACATGCTG CGCATCGGCC TGGATCAGCT
3361 CTACCAGAGC CTCGTGGACG GCAACTTCGA CGATGCTGCC TGGGCTCAAG GCCGCTGCGA
3421 TGCCATCGTC CGCAGCGGGG CCGGCACCGT CGGTCGCACA CAAATCGCTC GCCGGAGCGC
3481 AGCCGTATGG ACCGACGGCT GCGTCGAGGT GCTGGCCGAC AGCGGCAACC GCCGGCCCAG
3541 TACACGACCG CGCGCTAAGG AGGTAGGTCG AGTTTAAACT CTAGAACCGG TCATGGCCGC
3601 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGT TCGAACTAGA
3661 TGCTGTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG
3721 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG
3781 GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
3841 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
3901 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
3961 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
4021 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
4081 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
4141 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
4201 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
4261 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
4321 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
4381 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
4441 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
4501 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
4561 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
4621 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
4681 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCGGCCG
4741 CAAATGCTAA ACCACTGCAG TGGTTACCAG TGCTTGATCA GTGAGGCACC GATCTCAGCG
4801 ATCTGCCTAT TTCGTTCGTC CATAGTGGCC TGACTCCCCG TCGTGTAGAT CACTACGATT
4861 CGTGAGGGCT TACCATCAGG CCCCAGCGCA GCAATGATGC CGCGAGAGCC GCGTTCACCG
4921 GCCCCCGATT TGTCAGCAAT GAACCAGCCA GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT
4981 GCTACTTTGT CCGCCTCCAT CCAGTCTATG AGCTGCTGTC GTGATGCTAG AGTAAGAAGT
5041 TCGCCAGTGA GTAGTTTCCG AAGAGTTGTG GCCATTGCTA CTGGCATCGT GGTATCACGC
5101 TCGTCGTTCG GTATGGCTTC GTTCAACTCT GGTTCCCAGC GGTCAAGCCG GGTCACATGA
5161 TCACCCATAT TATGAAGAAA TGCAGTCAGC TCCTTAGGGC CTCCGATCGT TGTCAGAAGT
5221 AAGTTGGCCG CGGTGTTGTC GCTCATGGTA ATGGCAGCAC TACACAATTC TCTTACCGTC
5281 ATGCCATCCG TAAGATGCTT TTCCGTGACC GGCGAGTACT CAACCAAGTC GTTTTGTGAG
5341 TAGTGTATAC GGCGACCAAG CTGCTCTTGC CCGGCGTCTA TACGGGACAA CACCGCGCCA
5401 CATAGCAGTA CTTTGAAAGT GCTCATCATC GGGAATCGTT CTTCGGGGCG GAAAGACTCA
5461 AGGATCTTGC CGCTATTGAG ATCCAGTTCG ATATAGCCCA CTCTTGCACC CAGTTGATCT
5521 TCAGCATCTT TTACTTTCAC CAGCGTTTCG GGGTGTGCAA AAACAGGCAA GCAAAATGCC
5581 GCAAAGAAGG GAATGAGTGC GACACGAAAA TGTTGGATGC TCATACTCGT CCTTTTTCAA
5641 TATTATTGAA GCATTTATCA GGGTTACTAG TACGTCTCTC AAGGATAAGT AAGTAATATT
5701 AAGGTACGGG AGGTATTGGA CAGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG
5761 TGTTGGTTTT TTGTGTGAAT CGATAGTACT AACATACGCT CTCCATCAAA ACAAAACGAA
5821 ACAAAACAAA CTAGCAAAAT AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA GAACATTTCT
5881 CT