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pGL4.17[luc2/Neo] 載體信息

  • 產(chǎn)品貨號(hào)GS-1731
  • 銷售價(jià)格¥ 680
  • 包裝規(guī)格2μg質(zhì)?;?.5ml甘油菌
  • 載體抗性Ampicillin (氨芐青霉素)
  • 篩選標(biāo)記Neomycin (新霉素)
  • 載體大小5599 bp
  • 備注說明Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay.

pGL4.17[luc2/Neo] 載體說明

The pGL4.17[luc2/Neo] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.17[luc2/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.

pGL4.17[luc2/Neo] 載體圖譜

  • pGL4.17[luc2/Neo]質(zhì)粒圖譜/載體圖譜
  • pGL4.17[luc2/Neo]質(zhì)粒圖譜/載體圖譜

pGL4.17[luc2/Neo] 載體序列

0001 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC 
0061 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT 
0121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC 
0181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC 
0241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG 
0301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG 
0361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC 
0421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC 
0481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA 
0541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC 
0601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC 
0661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC 
0721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC 
0781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC 
0841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC 
0901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG 
0961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC 
1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG 
1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC 
1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC 
1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC 
1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT 
1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC 
1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC 
1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC 
1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG 
1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA 
1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC 
1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG 
1741 ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA 
1801 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT 
1861 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC 
1921 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA 
1981 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG 
2041 CTCTTCCGCT GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT 
2101 TAGCTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 
2161 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 
2221 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 
2281 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 
2341 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 
2401 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 
2461 TTTGCAAAAA GCTCGATTCT TCTGACACTA GCGCCACCAT GATCGAACAA GACGGCCTCC 
2521 ATGCTGGCAG TCCCGCAGCT TGGGTCGAAC GCTTGTTCGG GTACGACTGG GCCCAGCAGA 
2581 CCATCGGATG TAGCGATGCG GCCGTGTTCC GTCTAAGCGC TCAAGGCCGG CCCGTGCTGT 
2641 TCGTGAAGAC CGACCTGAGC GGCGCCCTGA ACGAGCTTCA AGACGAGGCT GCCCGCCTGA 
2701 GCTGGCTGGC CACCACCGGC GTACCCTGCG CCGCTGTGTT GGATGTTGTG ACCGAAGCCG 
2761 GCCGGGACTG GCTGCTGCTG GGCGAGGTCC CTGGCCAGGA TCTGCTGAGC AGCCACCTTG 
2821 CCCCCGCTGA GAAGGTTTCT ATCATGGCCG ATGCAATGCG GCGCCTGCAC ACCCTGGACC 
2881 CCGCTACCTG CCCCTTCGAC CACCAGGCTA AGCATCGGAT CGAGCGTGCT CGGACCCGCA 
2941 TGGAGGCCGG CCTGGTGGAC CAGGACGACC TGGACGAGGA GCATCAGGGC CTGGCCCCCG 
3001 CTGAACTGTT CGCCCGACTG AAAGCCCGCA TGCCGGACGG TGAGGACCTG GTTGTCACAC 
3061 ACGGAGATGC CTGCCTCCCT AACATCATGG TCGAGAATGG CCGCTTCTCC GGCTTCATCG 
3121 ACTGCGGTCG CCTAGGAGTT GCCGACCGCT ACCAGGACAT CGCCCTGGCC ACCCGCGACA 
3181 TCGCTGAGGA GCTTGGCGGC GAGTGGGCCG ACCGCTTCTT AGTCTTGTAC GGCATCGCAG 
3241 CTCCCGACAG CCAGCGCATC GCCTTCTACC GCTTGCTCGA CGAGTTCTTT TAATGATCTA 
3301 GAACCGGTCA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT 
3361 TGTGTGTTCG AACTAGATGC TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT 
3421 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA 
3481 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 
3541 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 
3601 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 
3661 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 
3721 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 
3781 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 
3841 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 
3901 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 
3961 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 
4021 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 
4081 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 
4141 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 
4201 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 
4261 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 
4321 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 
4381 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 
4441 TGGTCTGACA GCGGCCGCAA ATGCTAAACC ACTGCAGTGG TTACCAGTGC TTGATCAGTG 
4501 AGGCACCGAT CTCAGCGATC TGCCTATTTC GTTCGTCCAT AGTGGCCTGA CTCCCCGTCG 
4561 TGTAGATCAC TACGATTCGT GAGGGCTTAC CATCAGGCCC CAGCGCAGCA ATGATGCCGC 
4621 GAGAGCCGCG TTCACCGGCC CCCGATTTGT CAGCAATGAA CCAGCCAGCA GGGAGGGCCG 
4681 AGCGAAGAAG TGGTCCTGCT ACTTTGTCCG CCTCCATCCA GTCTATGAGC TGCTGTCGTG 
4741 ATGCTAGAGT AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC ATTGCTACTG 
4801 GCATCGTGGT ATCACGCTCG TCGTTCGGTA TGGCTTCGTT CAACTCTGGT TCCCAGCGGT 
4861 CAAGCCGGGT CACATGATCA CCCATATTAT GAAGAAATGC AGTCAGCTCC TTAGGGCCTC 
4921 CGATCGTTGT CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT CATGGTAATG GCAGCACTAC 
4981 ACAATTCTCT TACCGTCATG CCATCCGTAA GATGCTTTTC CGTGACCGGC GAGTACTCAA 
5041 CCAAGTCGTT TTGTGAGTAG TGTATACGGC GACCAAGCTG CTCTTGCCCG GCGTCTATAC 
5101 GGGACAACAC CGCGCCACAT AGCAGTACTT TGAAAGTGCT CATCATCGGG AATCGTTCTT 
5161 CGGGGCGGAA AGACTCAAGG ATCTTGCCGC TATTGAGATC CAGTTCGATA TAGCCCACTC 
5221 TTGCACCCAG TTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCGGGG TGTGCAAAAA 
5281 CAGGCAAGCA AAATGCCGCA AAGAAGGGAA TGAGTGCGAC ACGAAAATGT TGGATGCTCA 
5341 TACTCGTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTACTAGTAC GTCTCTCAAG 
5401 GATAAGTAAG TAATATTAAG GTACGGGAGG TATTGGACAG GCCGCAATAA AATATCTTTA 
5461 TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC ATACGCTCTC 
5521 CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA 
5581 GGTGCCAGAA CATTTCTCT