pGAPZ A
- 產(chǎn)品貨號GS-1991
- 銷售價(jià)格¥ 816
- 包裝規(guī)格2μg質(zhì)粒或0.5ml甘油菌
- 表達(dá)類型高
- 載體抗性Zeocin (博萊霉素)
- 載體大小2884 bp
- 啟動子GAP
- 5'測序引物pGAP-F:5?-GTCCCTATTTCAATCAATTGAA-3?
- 備注說明插入基因是必需包含起始密碼子ATG。
GAP啟動子(PGAP)的重組蛋白表達(dá)水平略高于AOX1啟動子。 pGAPZ A,B,和C載體(2.9 KB)和pGAPZα,B,和C(3.1 KB)載體使用GAP啟動子在畢赤酵母中穩(wěn)定表達(dá)重組蛋白。重組蛋白表達(dá)為為帶有C-末端myc抗原表位和C端His標(biāo)簽的融合蛋白。此外,pGAPZα表達(dá)的融合蛋白在N-端帶有釀酒酵母(Saccharomyces cerevisiae)的α-因子分泌信號肽。這兩個(gè)系列的載體都提供三個(gè)不同讀碼框的載體,以方便克隆的帶有C-端標(biāo)簽和/或N-末端分泌信號肽的載體中。 這些載體是基于顯性選擇標(biāo)記,Zeocin(博萊霉素)的篩選標(biāo)記可以同時(shí)使用于畢赤酵母和大腸桿菌。
0001 AGATCTTTTT TGTAGAAATG TCTTGGTGTC CTCGTCCAAT CAGGTAGCCA TCTCTGAAAT
0061 ATCTGGCTCC GTTGCAACTC CGAACGACCT GCTGGCAACG TAAAATTCTC CGGGGTAAAA
0121 CTTAAATGTG GAGTAATGGA ACCAGAAACG TCTCTTCCCT TCTCTCTCCT TCCACCGCCC
0181 GTTACCGTCC CTAGGAAATT TTACTCTGCT GGAGAGCTTC TTCTACGGCC CCCTTGCAGC
0241 AATGCTCTTC CCAGCATTAC GTTGCGGGTA AAACGGAGGT CGTGTACCCG ACCTAGCAGC
0301 CCAGGGATGG AAAAGTCCCG GCCGTCGCTG GCAATAATAG CGGGCGGACG CATGTCATGA
0361 GATTATTGGA AACCACCAGA ATCGAATATA AAAGGCGAAC ACCTTTCCCA ATTTTGGTTT
0421 CTCCTGACCC AAAGACTTTA AATTTAATTT ATTTGTCCCT ATTTCAATCA ATTGAACAAC
0481 TATTTCGAAA CGAGGAATTC ACGTGGCCCA GCCGGCCGTC TCGGATCGGT ACCTCGAGCC
0541 GCGGCGGCCG CCAGCTTGGG CCCGAACAAA AACTCATCTC AGAAGAGGAT CTGAATAGCG
0601 CCGTCGACCA TCATCATCAT CATCATTGAG TTTTAGCCTT AGACATGACT GTTCCTCAGT
0661 TCAAGTTGGG CACTTACGAG AAGACCGGTC TTGCTAGATT CTAATCAAGA GGATGTCAGA
0721 ATGCCATTTG CCTGAGAGAT GCAGGCTTCA TTTTTGATAC TTTTTTATTT GTAACCTATA
0781 TAGTATAGGA TTTTTTTTGT CATTTTGTTT CTTCTCGTAC GAGCTTGCTC CTGATCAGCC
0841 TATCTCGCAG CTGATGAATA TCTTGTGGTA GGGGTTTGGG AAAATCATTC GAGTTTGATG
0901 TTTTTCTTGG TATTTCCCAC TCCTCTTCAG AGTACAGAAG ATTAAGTGAG ACCTTCGTTT
0961 GTGCGGATCC CCCACACACC ATAGCTTCAA AATGTTTCTA CTCCTTTTTT ACTCTTCCAG
1021 ATTTTCTCGG ACTCCGCGCA TCGCCGTACC ACTTCAAAAC ACCCAAGCAC AGCATACTAA
1081 ATTTTCCCTC TTTCTTCCTC TAGGGTGTCG TTAATTACCC GTACTAAAGG TTTGGAAAAG
1141 AAAAAAGAGA CCGCCTCGTT TCTTTTTCTT CGTCGAAAAA GGCAATAAAA ATTTTTATCA
1201 CGTTTCTTTT TCTTGAAATT TTTTTTTTTA GTTTTTTTCT CTTTCAGTGA CCTCCATTGA
1261 TATTTAAGTT AATAAACGGT CTTCAATTTC TCAAGTTTCA GTTTCATTTT TCTTGTTCTA
1321 TTACAACTTT TTTTACTTCT TGTTCATTAG AAAGAAAGCA TAGCAATCTA ATCTAAGGGC
1381 GGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG
1441 AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC
1501 GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC
1561 TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG
1621 GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG
1681 TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC
1741 GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC
1801 TTCGTGGCCG AGGAGCAGGA CTGACACGTC CGACGGCGGC CCACGGGTCC CAGGCCTCGG
1861 AGATCCGTCC CCCTTTTCCT TTGTCGATAT CATGTAATTA GTTATGTCAC GCTTACATTC
1921 ACGCCCTCCC CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA
1981 GGTCCCTATT TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT
2041 TTTTCTTTTT TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT
2101 TGAGAAGGTT TTGGGACGCT CGAAGGCTTT AATTTGCAAG CTGGAGACCA ACATGTGAGC
2161 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
2221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
2281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
2341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
2401 TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
2461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
2521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
2581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
2641 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
2701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
2761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
2821 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCATGA
2881 GATC