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pET-50b(+) 載體信息

  • 產(chǎn)品貨號GS-1235
  • 銷售價格¥ 680
  • 包裝規(guī)格2μg質(zhì)粒或0.5ml甘油菌
  • 表達類型
  • 載體抗性Kanamycin (卡那霉素)
  • 載體大小6733 bp
  • 5'測序引物T7
  • 3'測序引物T7t
  • 備注說明Same as pET47 but also has Nterm Nus Tag; contains HRV 3C Protease cleavage site for fusion tag removal at low temperatures; Cterm thrombin cleavage site

pET-50b(+) 載體說明

The pET-50b(+) vector carries N-terminal Nus?Tag? and His?Tag? coding sequences followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specifi c for cleavage of the sequence LEVLFQ↓GP (1), and is active at low temperatures (2). pET-50b(+) also contains an optional C-terminal thrombin recognition site followed by an S?Tag? coding sequence. Unique restriction sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with the helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the AS S?Tag 18mer Primer (Cat. No. 71262-3).

pET-50b(+) 載體圖譜

  • pET-50b(+)質(zhì)粒圖譜/載體圖譜
  • pET-50b(+)質(zhì)粒圖譜/載體圖譜