443143416pET-44 EK/LIC
- 產(chǎn)品貨號GS-1940
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 表達(dá)類型高
- 載體抗性Ampicillin (氨芐青霉素)
- 載體大小7331 bp
- 備注說明For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
The pET-44 Ek/LIC Vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal His?Tag?,Nus?Tag? and S?Tag? sequences. Using specifically designed primers for amplification and the pET-44 Ek/LIC Vector Kit (Cat. No. 71144-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the COLIDOWN Primer (Cat. No. 70845-3). Vector encoded sequences can be completely removed when cloning into the Ek/LIC site (as shown below left) by cleaving the fusion protein with enterokinase.
0001 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
0061 GGGGTTATGC TAGTTATTGC TCAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
0121 AAAGAGTTTG TAGAAACGCA AAAAGGCCAT CCGTCAGGAT GGCCTTCTGC TTAATTTGAT
0181 GCCTGGCAGT TTATGGCGGG CGTCCTGCCC GCCACCCTCC GGGCCGTTGC TTCGCAACGT
0241 TCAAATCCGC TCCCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA
0301 TAAAACGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC
0361 CCTACTCTCG CATGGGGAGA CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG
0421 TTCGGCATGG GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAATT CTGTTTTATC
0481 AGCCTAGGAA CGCCCAACTT AATTAACATT AGTGGTGGTG GTGGTGGTGC TCGAGATCCT
0541 CTGGGTCTTC AGGAGCGAGT TCTGGCTGGC TTGCACGTGT ATACAGCTGT GCGGCCGCAA
0601 GCTTCGAACG CGTATCGATG GTACCGTCGA CGTCCTGCAG GCGCGCCTGT ACAGAATTCG
0661 GATCCACGAG CTCAGATATC GTTGAGGAGA AGCCCGGACT CTTGTCGTCG TCATCAATCG
0721 TACCAGAACC CGCGCTGCCC CGGGGGACCA GACCAGTTGG CGGTGGCGAG TCCATGTGCT
0781 GGCGTTCAAA TTTCGCAGCA GCGGTTTCTT TACCCGCGGA GTGATGGTGA TGGTGATGAC
0841 CAGAACCACT AGTCGCTTCG TCACCGAACC AGCAAATATT ACGGGCAGCC ATAATCAGTG
0901 CTCCGGCTTT TTCGTCGGTC AACCCTTCGA TATCAGCCAG ATCATCAATG CCCTGTTCGG
0961 CGAGATCTTC CAGCGTACAA ACGCCACGGG CGGCCAGTTT GAATGCCAAA TCACGATCTA
1021 CCCCTTCAAG GTTCAGCAGA TCGTCAGCCG GTTTGTTATC ACCGAGGCTT TCTTCCTGGG
1081 CCTGTGCAAT GGTGGCCAGT GCATTTTTAG CACGCTCGCG CAGTGCTTCA ACGGTCGGCT
1141 CATCAAGGCC TTCGATTTCC AACAGCTCTT TCATCGGCAC ATAGGCCAAT TCTTCCAGCG
1201 TCGAGAAGCC TTCTTCTACC AGAACAGTCG CGAAGTCTTC GTCGATGTCG AGATATTTGG
1261 TGAAGGTGTC GATCGCTGCG TGCGCTTCCG CCTGATGCTT AGCTTGCAGG TCGTCAACGG
1321 TCATCACGTT GAGTTCCCAA CCGCTCAGTT GCGAAGCCAG ACGCACGTTC TGACCGTTAC
1381 GGCCAATCGC CTGCGCCAGA TTACCGGCTT CAACGGCGAT GTCCATGGTG TGTTTATCTT
1441 CATCCACCAC GATAGAAGCA ACGTCTGCCG GTGCCATTGC GTTAATCACG AACTGCGCCG
1501 GGTTATCATC CCACAGGACG ATATCGATAC GCTCGCCACC CAGTTCAGTA GACACCGCCT
1561 GAACACGCGC GCCACGCATA CCTACGCAAG CACCTACCGG ATCGATACGT TTATCGTTGG
1621 TTTTCACCGC GATTTTCGCA CGAGAACCCG GATCGCGAGC CGCTGCTTTA ATTTCAATCA
1681 CTTCTTCGCC GATTTCTGGC ACTTCAATAC GGAACAGTTC GATCAGCATT TCCGGCTTGG
1741 AACGAGTGAC GAACAGTTGC GCGCCACGCG CTTCCGGGCG AACGGAATAG AGCACGCCAC
1801 GAACGCGGTC GCCAGGGCGG AAGTTTTCAC GCGGCAGCAT ATCTTCGCGC AGGATCACGG
1861 CTTCAGCGTT GTTGCCCAGA TCCAGAGAGA TGTTGTCGCG GTTTACTTTT TTCACCACGC
1921 CGGTGATGAT TTCACCTTCG TGTTCACGGA ACTGATCAAC CACCATCGCA CGTTCGGCTT
1981 CACGCACTTT CTGCACGATA ACCTGTTTTG CCGTCTGGGT AGTGATACGG TCAAAGGTAA
2041 CAGACTCAAT CTGATCTTCA ACGTAATCGC CCAGGTTCAG GCTTTCATCT TCATAACGTG
2101 CGGCTTCAAG GGTGATTTCC TTGGTCGGCT GGGTGACTTC ATCAACAACT AACCAGCGAC
2161 GGAAAGTGTC AAAATCACCG CTTTTGCGAT CGATCTGTAC GCGGACGTCG ATCTCTTGTT
2221 CATATTTTTT CTTTGTTGCT GTCGCCAGCG CGCTTTCCAA TGCTTCGAAA ATCTTCTCGC
2281 GAGGTAGCGC CTTTTCATTG GATACGGCTT CAACTACAGC CAAAATTTCT TTGTTCATAC
2341 TAGAATGGTG ATGGTGGTGA TGGCTGCTGC CCATATGTAT ATCTCCTTCT TAAAGTTAAA
2401 CAAAATTATT TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT
2461 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG TGGCCGGCAT
2521 CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG ATGGGGAAGA
2581 TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG TGGCAGGCCC
2641 CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT
2701 GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA
2761 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC
2821 GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG
2881 CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG
2941 TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA
3001 ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA
3061 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC
3121 TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG
3181 CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG
3241 ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG
3301 TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG
3361 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA
3421 GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT CGCAATCAAA
3481 TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT CAACAAACCA
3541 TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG
3601 CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG
3661 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA ACCACCATCA
3721 AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG
3781 GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA AAAACCACCC
3841 TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG
3901 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG
3961 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC
4021 TCCTTCCGGT GGGCGCGGGG CATGACTAGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG
4081 CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG
4141 TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT
4201 TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG
4261 ATCTGCATCG CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG
4321 CGCTGGCATT GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT
4381 TTACCCTCAC AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC
4441 ATCCTCTCTC GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG
4501 GCATCAGTGA CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG
4561 ACATTAACGC TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT
4621 GAATCGCTTC ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT
4681 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG
4741 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC
4801 GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT
4861 CAGAGCAGAT TGTACTGAGA GTGCACCATA TATGCGGTGT GAAATACCGC ACAGATGCGT
4921 AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
4981 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GACCTATTTG TTTATTTTTC TAAATACATT
5821 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATCA TGATTGAAAA
5881 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
5941 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG
6001 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT
6061 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG
6121 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG
6181 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
6241 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG
6301 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA
6361 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC
6421 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT
6481 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
6541 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG
6601 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA
6661 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG
6721 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT
6781 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
6841 AATCTCATGC CATCGTGCCG CCAGAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT
6901 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
6961 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC
7021 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG
7081 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC
7141 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG
7201 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG
7261 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
7321 CCCATTCGCC A