The pET-42 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST?Tag? protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Factor Xa.

0001 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 
0061 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 
0121 TGTTTAGCAG CCTAGGTATT AATCAATTAG TGGTGGTGGT GGTGGTGGTG GTGCTCGAGT 
0181 GCGGCCGCAA GCTTGTCGAC GGAGCTCGCC TGCAGGCGCG CCAAGGCCTG TACAGAATTC 
0241 GGATCCCCGA TATCCCATGG ACCCGCGTCC CTCAATACCG GAGCCACCAC CGGTACCCAG 
0301 ATCTGGGCTG TCCATGTGCT GGCGTTCGAA TTTAGCAGCA GCGGTTTCTT TCATACCAAT 
0361 TGCAGTACTA CCGCGTGGCA CCAGACCCGC GGAGTGATGG TGATGGTGAT GACCAGAACC 
0421 ACTAGTTGAA CCATCCGATT TTGGAGGATG GTCGCCACCA CCAAACGTGG CTTGCCAGCC 
0481 CTGCAAAGGC CATGCTATAT ACTTGCTGGA TTTCAAGTAC TTATCAATTT GTGGGATAGC 
0541 TTCAATACGT TTTTTAAAAC AAACTAATTT TGGGAACGCA TCCAGGCACA TTGGGTCCAT 
0601 GTATAAAACA ACATCAAGAG CGTCATACAA CATGAAGTCA GGATGGGTTA CATGATCACC 
0661 ATTTAAATAT GTTTTATGAC ATAAACGATC TTCGAACATT TTCAGCATTT CAGGTAGCTT 
0721 GCTAAGAAAA TCAACTTTGA GAGTTTCAAA GTCTTTACTA TATGCAATTC TCGAAACACC 
0781 GTATCTAATA TCCAAAACCG CTCCTTCAAG CATTGAAATC TCTGCACGCT CTTTTGGACA 
0841 ACCACCCAAC ATGTTGTGCT TGTCAGCTAT ATAACGTATG ATGGCCATAG ACTGTGTTAA 
0901 TTTAACATCA CCATCAATAT AATAAGGAAG ATTGGGAAAC TCCAAACCCA ATTCAAACTT 
0961 TTTGTTTCGC CATTTATCAC CTTCATCGCG CTCATACAAA TGCTCTTCAT ATTTTTCTTC 
1021 AAGATATTCC AAAAGAAGTC GAGTGGGTTG CACAAGGCCC TTAATTTTCC AATAACCTAG 
1081 TATAGGGGAC ATATGTATAT CTCCTTCTTA AAGTTAAACA AAATTATTTC TAGAGGGGAA 
1141 TTGTTATCCG CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGCGGG ATCGAGATCG 
1201 ATCTCGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT 
1261 GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC 
1321 ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC 
1381 GCCATCTCCT TGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA 
1441 CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGAGATCC CGGACACCAT 
1501 CGAATGGCGC AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG 
1561 GGTGGTGAAT GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA 
1621 TCAGACCGTT TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA 
1681 AGTGGAAGCG GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC 
1741 GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC ACGCGCCGTC 
1801 GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC 
1861 GATGGTAGAA CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC TTCTCGCGCA 
1921 ACGCGTCAGT GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA 
1981 AGCTGCCTGC ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA 
2041 CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT 
2101 GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGTCTGCG 
2161 TCTGGCTGGC TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG CGGAACGGGA 
2221 AGGCGACTGG AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT 
2281 CGTTCCCACT GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT 
2341 TACCGAGTCC GGGCTGCGCG TTGGTGCGGA CATCTCGGTA GTGGGATACG ACGATACCGA 
2401 AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA CAGGATTTTC GCCTGCTGGG 
2461 GCAAACCAGC GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA AGGGCAATCA 
2521 GCTGTTGCCC GTCTCACTGG TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC 
2581 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 
2641 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG GCACCGGGAT 
2701 CTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA 
2761 TGACTAGCAT GATCGTGCTC CTGTCGTTGA GGACCCGGCT AGGCTGGCGG GGTTGCCTTA 
2821 CTGGTTAGCA GAATGAATCA CCGATACGCG AGCGAACGTG AAGCGACTGC TGCTGCAAAA 
2881 CGTCTGCGAC CTGAGCAACA ACATGAATGG TCTTCGGTTT CCGTGTTTCG TAAAGTCTGG 
2941 AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT CTGCATCGCA GGATGCTGCT 
3001 GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG CTGGCATTGA CCCTGAGTGA 
3061 TTTTTCTCTG GTCCCGCCGC ATCCATACCG CCAGTTGTTT ACCCTCACAA CGTTCCAGTA 
3121 ACCGGGCATG TTCATCATCA GTAACCCGTA TCGTGAGCAT CCTCTCTCGT TTCATCGGTA 
3181 TCATTACCCC CATGAACAGA AATCCCCCTT ACACGGAGGC ATCAGTGACC AAACAGGAAA 
3241 AAACCGCCCT TAACATGGCC CGCTTTATCA GAAGCCAGAC ATTAACGCTT CTGGAGAAAC 
3301 TCAACGAGCT GGACGCGGAT GAACAGGCAG ACATCTGTGA ATCGCTTCAC GACCACGCTG 
3361 ATGAGCTTTA CCGCAGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 
3421 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 
3481 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA 
3541 GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 
3601 GCACCATATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 
3661 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 
3721 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 
3781 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 
3841 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 
3901 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 
3961 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 
4021 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 
4081 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 
4141 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 
4201 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 
4261 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 
4321 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 
4381 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 
4441 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 
4501 TTGGTCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 
4561 CCATATTCAA CGGGAAACGT CTTGCTCTAG GCCGCGATTA AATTCCAACA TGGATGCTGA 
4621 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 
4681 ATTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 
4741 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 
4801 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 
4861 CGGGAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 
4921 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 
4981 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 
5041 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 
5101 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 
5161 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 
5221 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 
5281 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 
5341 GTTTCATTTG ATGCTCGATG AGTTTTTCTA AGAATTAATT CATGAGCGGA TACATATTTG 
5401 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
5461 CTGAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT 
5521 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG 
5581 AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT 
5641 CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC 
5701 CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA 
5761 GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA 
5821 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA 
5881 CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC CCATTCGCCA