443143416pcDNA3.1/Zeo(+)
- 產(chǎn)品貨號(hào)GS-1012
- 銷(xiāo)售價(jià)格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 表達(dá)類(lèi)型高
- 載體抗性Ampicillin (氨芐青霉素)
- 篩選標(biāo)記Zeocin 博來(lái)霉素
- 載體大小5015 bp
- 啟動(dòng)子CMV
- 5'測(cè)序引物pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
- 3'測(cè)序引物pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
- 備注說(shuō)明Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.
This pcDNA?3.1/Zeo(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin? selectable marker and a forward-orientation multiple cloning site.
The pcDNA?3.1 Expression Vector Family
Three untagged versions of pcDNA?3.1 (available separately), each with a different selectable marker (Geneticin?, Zeocin?, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in?E. coli
