443143416pBudCE4.1
- 產(chǎn)品貨號(hào)GS-1019
- 銷售價(jià)格￥ 1020
- 包裝規(guī)格2μg質(zhì)粒或0.5ml甘油菌
- 載體抗性Zeocin (博萊霉素)
- 載體大小4595 bp
- 啟動(dòng)子EF-1α, CMV
- 5'測(cè)序引物CMVPro-F: 5'd[TAATACGACTCACTATAGGG]
- 3'測(cè)序引物3'EF1aPro-F:5'd[TCAAGCCTCAGACAGTGGTTC]3'
- 備注說(shuō)明能夠利用該載體同時(shí)表達(dá)兩種基因
The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in the cell. This can eliminate variable expression due to differences in gene copy number. pBudCE4.1 provides expression cassettes withthe following features:
- The CMV promoter for high-level transcription of genes with an optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification
- The human EF-1α promoter for high-level expression of genes with an optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
- The Sh ble (ZeoR) gene for efficient selection in both mammalian cells and E. coliwith the selection agent Zeocin?
0001 GCGCGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG
0061 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
0121 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
0181 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGACTATTT ACGGTAAACT GCCCACTTGG
0241 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT
0301 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
0361 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC
0421 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA
0481 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT
0541 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC
0601 TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA
0661 CCCAAGCTTG CATTCCTGCA GGTCGACATC GATCTTAAGC AGTACTTCTA GAGGATCCGA
0721 ACAAAAACTC ATCTCAGAAG AGGATCTGAA TATGCATACC GGTCATCATC ACCATCACCA
0781 TTGAGTTTGA TCCCCGGGAA TTCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
0841 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
0901 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTGGGGTGG GCGAAGAACT CCAGCATGAG
0961 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT
1021 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTAGCACG TGTCAGTCCT GCTCCTCGGC
1081 CACGAAGTGC ACGCAGTTGC CGGCCGGGTC GCGCAGGGCG AACTCCCGCC CCCACGGCTG
1141 CTCGCCGATC TCGGTCATGG CCGGCCCGGA GGCGTCCCGG AAGTTCGTGG ACACGACCTC
1201 CGACCACTCG GCGTACAGCT CGTCCAGGCC GCGCACCCAC ACCCAGGCCA GGGTGTTGTC
1261 CGGCACCACC TGGTCCTGGA CCGCGCTGAT GAACAGGGTC ACGTCGTCCC GGACCACACC
1321 GGCGAAGTCG TCCTCCACGA AGTCCCGGGA GAACCCGAGC CGGTCGGTCC AGAACTCGAC
1381 CGCTCCGGCG ACGTCGCGCG CGGTGAGCAC CGGAACGGCA CTGGTCAACT TGGCCATGGT
1441 TTAGTTCCTC ACCTTGTCGT ATTATACTAT GCCGATATAC TATGCCGATG ATTAATTGTC
1501 AACACGTGCT GATCAGATCC GAAAATGGAT ATACAAGCTC CCGGGAGCTT TTTGCAAAAG
1561 CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC AGAGGCGGCC
1621 TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG
1681 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG
1741 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT
1801 GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT
1861 CCACACCCTC GTCGAGCTAG CTTCGTGAGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC
1921 ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG
1981 AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA
2041 GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG
2101 GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC
2161 GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTCCAGTACG TGATTCTTGA
2221 TCCCGAGCTG GAGCCAGGGG CGGGCCTTGC GCTTTAGGAG CCCCTTCGCC TCGTGCTTGA
2281 GTTGAGGCCT GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC
2341 TGTCTCGCTG CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG
2401 CTTTTTTTCT GGCAAGATAG TCTTGTAAAT GCGGGCCAGG ATCTGCACAC TGGTATTTCG
2461 GTTTTTGGGC CCGCGGCCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG
2521 CGGGGCCTGC GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT
2581 GCTCTGGTGC CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC
2641 CGGTCGGCAC CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TCCAGGGGGC
2701 TCAAAATGGA GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA
2761 AGGGCCTTTC CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC
2821 AGGCACCTCG ATTAGTTCTG GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG
2881 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG
2941 CACTTGATGT AATTCTCGTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC
3001 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGTGGT
3061 CGCGGCCGCT TCGAAGGTAC CAGCACAGTG GACTCGAGAG ATCTGGCCGG CTGGGCCCGT
3121 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
3181 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG TGGCGGTAAT
3481 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
3541 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
3601 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
3661 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
3721 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
3781 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
3841 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
3901 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
3961 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
4021 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
4081 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
4141 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
4201 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACATTAA CCTATAAAAA
4261 TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
4321 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA
4381 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC
4441 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATATGCGGT GTGAAATACC GCACAGATGC
4501 GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA
4561 GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCA