443143416pBS185 CMV-Cre
- 產(chǎn)品貨號(hào)GS-1314
- 銷(xiāo)售價(jià)格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 載體抗性Ampicillin
- 載體大小6919 bp
BS185 carries the wt cre gene under the control of the major immediate early promoter and enhancer of human cytomegalovirus (hCMV). This is a strong promoter that gives fairly generalized expression. However, it is not a strong promoter in embryonic stem (ES) cells. The construct also carries the mouse metallothionein MT-I region including the polyadenylation site and several introns. For higher level expression in general and for better expression in ES cells plasmid pBS513 is superior. The sequence shown includes cre and the metallothionein region.
0001 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
0061 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
0121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
0181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CATGATTACG AATTCGAGCT
0241 CGGTACCCTC GACCAATTCC TGGCCTTCCT GCTGCTACAT CTCAAATATC TTGATTACAG
0301 GCAGGACCCA CGCTCTTTAT CACTTTTTTT TCCATCTTTT TTTTTTTGGA TATTTTCTTC
0361 GTTTATATTT CAAATGCTAT CCCAAAAGTC CCCTATACCC CACCCCTACC CCCACCCCGC
0421 TCCCCTACCC ACTCACTCCC ACTTCTTGGC CCTGGCTTTC CCCTGAACTG GGGCATATAG
0481 TTTGCTAGAC CAAGGGTCCT CTCTTCCCAG TGATGGCCAA CTAGGCCATC TTCTGCTACA
0541 TACGAAGCTA GATACACGAG TTCAGGGGGT ACTGGTTAGT TCAGATTGCT GTTCCACCTA
0601 TAGGGTTGCA GATCCCTTTA GCTCCTTGGG TACTTTCTCT AGCTCCTCCA TTGGGGGCCC
0661 TGTGTTCCAT CCAATAGCTG ACTGTGAGCA TCCACTTCTG TGTTTGCTGG GCACTGGCAA
0721 AGCCTCACAG GAGACAGCTT TATCAGGGTC CTTTCAGCAA AATCTTGATG GCATATGCAA
0781 TAGTGTCTGC ATTTGGTGGC TGAATAGGAG ATGGCCTGGT GACACAGTCA CTCCTGTAAT
0841 CCCAGGTACT TGGGAAGCTG AGGCAGGAGA ATCAAAAACT CAAGGTCAGA CTGGACTGCA
0901 GAATGAGTTC TAGGACAAGG TAAGCAACTT GTGTCAAAAC AGCCAAGAAA GCCCCTCTGG
0961 AATCTAGTTC AGTGTTAGTC CCTGCCTAGC ATACGAGACA CCCTATGTTA ATTTTCATAC
1021 CCACCTCCTT ATACCCAACA ATGTGAGAGG AAACCAGAAT CCAAAGAGAA TATAAATGAT
1081 AAGATTTTCT GTGCTTCCAG GATGGGACAT CAAGTCTTCC AGGACCACCC TCCCTCCCTC
1141 CCTTCCCCTA ACACAAGACC TGACCAGGCA CACAGCTGTT TTCCATGAGG AAGCTTATGG
1201 GATAGCTTGG CTTTACCCAA AGACATTACC CCTCTGTGAG GGCCAGAGGC GGGAGGTAAA
1261 GGCACGAGGG GGTCCAAAAC CAGATCCCCA GACTATCAAG TCACCCCAGT TTCTTATTGC
1321 CAAACCACAC CAAGAAAACC AGAGTTAGAC TCAAACAGGC TTTTATTATT CACGTACTCG
1381 GTAGAAAACG GGGGTTTAGT AAACAGGGTG GAACTGTATA GGAAGACGCT GGGTTGGTCC
1441 GATACTATTT ACACGTGGTG GCAGCGCTGT TCGTCACATC AGGCACAGCA CGTGCACTTG
1501 TCCGCGGCGC CTTTGCAGAC ACAGCCCTGG GCACATTTGG AGCAGCCCAC GGGACAGCAG
1561 GAGCAGCAGC CTAGAAAGGA GGAGGAAGAG GTCAGCCTGG CTGAAGACCT CTGCTTCCTG
1621 TCCCCAACAG AGAAGACCTC GGTTTTTTTA AATAGTTCTC TGTATAGTTC TGTCCCCGAG
1681 ACAATACAAT GGCCTCCGGG AAGCAAAGGA CATTTCTCAG AGCCAGTTTT GTAGGAGTTC
1741 CCCGCCCCTA GCCTTAGCCG CCACCCAAGG TGTCCCAACT CACTCTTCTT GCAGGAGGTG
1801 CACTTGCAGT TCTTGCAGGC GCAGGAGCTG GTGCAAGTGC AGGAGCCGCC TGGGGAGGAG
1861 AAAGAAGACA GCATGAGGGA GGCAGCATTA CAGCAGTGGC CAACACCACG AGTCCCGGCT
1921 CAGTTCACTA AGTCCTCCTC GGAGCTGCAG GGAGCCTAGC CCCACTTTTC TCCTCACAGG
1981 TTAAGTCAGG GATTATGTCT TTGAGTCCCA AGACATAAAG GTCCTTCACC TCTTTCTTAA
2041 TTCAAGCAAT TCACAGTAAG TTTAGTAATG CCTGGGACTC AACCCAGATG TCCCCACTAA
2101 CTGGGTGATC AGGAACCGGA CATTTGCGCG TCTAAATCCT AGCCCCAACC CCGCCAAAGG
2161 ACCCGTTCGA CAATACTCGT AACTAATGAA GGCTTTTGCA AGAGCGGATT CTAGAAAGAA
2221 CCGGAATGAA TCGCCAACTA AAGGTGCCTA TTCCCGCCAA GCCTCTACAA CTCGGGGATT
2281 CCGCTCTGCG GTCCCAACTT GGTATTCTAA AGACCAAGGA TCGGGAGTCT TACCGGTGGA
2341 GCAGGAGCAG TTGGGGTCCA TTCCGAGATC CCTCGACAAT TCCAACCTTA CCCAAGAGTT
2401 CGCCAAACTC AGACATCACT TTAGCAAAAC CGCGCCGTGC TTCTTCCTCG GTGGCATTCA
2461 TCACGAAATG TTCAGCACTA CGCATACTTT TGACAGGAAA CGCAACGGAT ATTGAGTCAA
2521 TATCAGGCAT TCTATCGCTC AGCTTTACAG TGACAATGAC GGCTGGCGAC TGAATATTAG
2581 TGCTTACAGA CAGCACTACA TATTTTCCGT CGATGTTGAA ATCCTTTCTC ATATGTCACC
2641 ATAAATATCA AATAATTATA GCAATCATTT ACGCGTTAAT GGCTAATCGC CATCTTCCAG
2701 CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG ATATAGTTCA TGACAATATT
2761 TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT GAAACTCCAG CGCGGGCCAT
2821 ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG GCCAGGTATC TCTGACCAGA
2881 GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA AAAATCCCTT CCAGGGCGCG
2941 AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA TTTTTTCTGA CCCGGCAAAA
3001 CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA ATCCATCGCT CGACCAGTTT
3061 AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG CTAACCAGCG TTTTCGTTCT
3121 GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG ATATCTTTAA CCCTGATCCT
3181 GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAGCAATC CCCAGAAATG CCAGATTACG
3241 TATATCCTGG CAGCGATCGC TATTTTCCAT GAGTGAACGA ACCTGGTCGA AATCAGTGCG
3301 TTCGAACGCT AGAGCCTGTT TTGCACGTTC ACCGGCATCA ACGTTTTCTT TTCGGATCCG
3361 CCGCATAACC AGTGAAACAG CATTGCTGTC ACTTGGTCGT GGCAGCCCGG ACCGACGATG
3421 AAGCATGTTT AGCTGGCCCA AATGTTGCTG GATAGTTTTT ACTGCCAGAC CGCGCGCCTG
3481 AAGATATAGA AGATAATCGC GAACATCTTC AGGTTCTGCG GGAAACCATT TCCGGTTATT
3541 CAACTTGCAC CATGCCGCCC ACGACCGGCA AACGGACAGA AGCATTTTCC AGGTATGCTC
3601 AGAAAACGCC TGGCGATCCC TGAACATGTC CATCAGGTTC TTGCGAACCT CATCACTCGT
3661 TGCATCGACC GGTAATGCAG GCAAATTTTG GTGTACGGTC AGTAAATTGG ACATTTCACA
3721 CTCAGATAAT GGTTTTAAGT AAAGTGTACA GGATCGGCTC TGCCCCTCGA GGGGATCCTC
3781 TAGAGTCGGT GTCTTCTATG GAGGTCAAAA CAGCGTGGAT GGCGTCTCCA GGCGATCTGA
3841 CGGTTCACTA AACGAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT
3901 TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA
3961 AACAAACTCC CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC
4021 TATCCACGCC CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT
4081 ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG
4141 GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA
4201 TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC
4261 CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG
4321 GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC CATATATGGG
4381 CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC TAGTCAATAA TCAATGTCAA
4441 CATGGCGGTA ATGTTGGACA TGAGCCAATA TAAATGTACA TATTATGATA TGGATACAAC
4501 GTATGCAATG GGCCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC
4561 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT
4621 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG
4681 CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC
4741 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA
4801 CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG
4861 ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA
4921 CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT
4981 TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
5041 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
5101 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT
5161 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT
5221 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC
5281 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA
5341 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC
5401 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC
5461 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC
5521 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG
5581 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC
5641 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC
5701 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT
5761 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA
5821 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC
5881 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG
5941 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA
6001 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
6061 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
6121 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
6181 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
6241 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT
6301 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
6361 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
6421 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
6481 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
6541 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
6601 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
6661 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
6721 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
6781 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT
6841 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA
6901 GTGAGCGAGG AAGCGGAAG