pBlueBacHis2 B
- 產(chǎn)品貨號(hào)GS-1174
- 銷(xiāo)售價(jià)格¥ 680
- 包裝規(guī)格2μg質(zhì)?;?.5ml甘油菌
- 表達(dá)類(lèi)型高
- 載體抗性Ampicillin (氨芐青霉素)
- 載體大小4853 bp
- 啟動(dòng)子Polyhedrin
- 5'測(cè)序引物polyhedrin forward
pBlueBacHis2 A (4863 bp), pBlueBacHis2 B (4853 bp), and pBlueBacHis2 C (4861 bp) are baculovirus transfer vectors that contain the Xpress?N-terminal tag for expression and purification of recombinant fusion proteins. The pBlueBacHis2 vector is provided in three different versions for simplified cloning of your gene in-frame with the Xpress?tag. The vectors contain sequences homologous to the lacZ gene and ORF1629 sequences in Bac-N-Blue?linear DNA, allowing production of blue, recombinant plaques. Expression of your recombinant fusion protein is driven by the polyhedrin promoter.
0001 GATATCATGG AGATAATTAA AATGATAACC ATCTCGCAAA TAAATAAGTA TTTTACTGTT
0061 TTCGTAACAG TTTTGTAATA AAAAACCTAT AAATATGCCG CGGGGTTCTC ATCATCATCA
0121 TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC TGTACGACGA
0181 TGACGATAAG GATCCGAGCT CGAGATCTGC AGCTGGTACC ATGGAATTCG AAGCTTGGAG
0241 TCGACTCTGC TGAAGAGGAG GAAATTCTCC TTGAAGTTTC CCTGGTGTTC AAAGTAAAGG
0301 AGTTTGCACC AGACGCACCT CTGTTCACTG GTCCGGCGTA TTAAAACACG ATACATTGTT
0361 ATTAGTACAT TTATTAAGCG CTAGATTCTG TGCGTTGTTG ATTTACAGAC AATTGTTGTA
0421 CGTATTTTAA TAATTCATTA AATTTATAAT CTTTAGGGTG GTATGTTAGA GCGAAAATCA
0481 AATGATTTTC AGCGTCTTTA TATCTGAATT TAAATATTAA ATCCTCAATA GATTTGTAAA
0541 ATAGGTTTCG ATTAGTTTCA AACAAGGGTT GTTTTTCCGA ACCGATGGCT GGACTATCTA
0601 ATGGATTTTC GCTCAACGCC ACAAAACTTG CCAAATCTTG TAGCAGCAAT CTAGCTTTGT
0661 CGATATTCGT TTGTGTTTTG TTTTGTAATA AAGGTTCGAC GTCGTTCAAA ATATTATGCG
0721 CTTTTGTATT TCTTTCATCA CTGTCGTTAG TGTACAATTG ACTCGACGTA AACACGTTAA
0781 ATAAAGCCTG GACATATTTA ACATCGGGCG TGTTAGCTTT ATTAGGCCGA TTATCGTCGT
0841 CGTCCCAACC CTCGTCGTTA GAAGTTGCTT CCGAAGACGA TTTTGCCATA GCCACACGAC
0901 GCCTATTAAT TGTGTCGGCT AACACGTCCG CGATCAAATT TGTAGTTGAG CTTTTTGGAA
0961 TTATTTCTGA TTGCGGGCGT TTTTGGGCGG GTTTCAATCT AACTGTGCCC GATTTTAATT
1021 CAGACAACAC GTTAGAAAGC GATGGTGCAG GCGGTGGTAA CATTTCAGAC GGCAAATCTA
1081 CTAATGGCGG CGGTGGTGGA GCTGATGATA AATCTACCAT CGGTGGAGGC GCAGGCGGGG
1141 CTGGCGGCGG AGGCGGAGGC GGAGGTGGTG GCGGTGATGC AGACGGCGGT TTAGGCTCAA
1201 ATTGTCTCTT TCAGGCAACA CAGTCGGCAC CTCAACTATT GTACTGGTTT CGGGCGTATG
1261 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
1321 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
1381 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
1441 GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
1501 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
1561 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
1621 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
1681 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
1741 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
1801 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
1861 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
1921 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
1981 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
2041 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
2101 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
2161 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
2221 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
2281 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
2341 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
2401 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
2461 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
2521 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
2581 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
2641 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
2701 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
2761 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
2821 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
2881 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
2941 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
3001 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
3061 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
3121 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
3181 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
3241 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
3301 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
3361 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
3421 GTCAGTGAGC GAGGAAGCAT CCTGCACCAT CGTCTGCTCA TCCATGACCT GACCATGCAG
3481 AGGATGATGC TCGTGACGGT TAACGCCTCG AATCAGCAAC GGCTTGCCGT TCAGCAGCAG
3541 CAGACCATTT TCAATCCGCA CCTCGCGGAA ACCGACATCG CAGGCTTCTG CTTCAATCAG
3601 CGTGCCGTCG GCGGTGTGCA GTTCAACCAC CGCACGATAG AGATTCGGGA TTTCGGCGCT
3661 CCACAGTTTC GGGTTTTCGA CGTTCAGACG TAGTGTGACG CGATCGGTAT AACCACCACG
3721 CTCATCGATA ATTTCACCGC CGAAAGGCGC GGTGCCGCTG GCGACCTGCG TTTCACCCTG
3781 CCATAAAGAA ACTGTTACCC GTAGGTAGTC ACGCAACTCG CCGCACATCT GAACTTCAGC
3841 CTCCAGTACA GCGCGGCTGA AATCATCATT AAAGCGAGTG GCAACATGGA AATCGCTGAT
3901 TTGTGTAGTC GGTTTATGCA GCAACGAGAC GTCACGGAAA ATGCCGCTCA TCCGCCACAT
3961 ATCCTGATCT TCCAGATAAC TGCCGTCACT CCAACGCAGC ACCATCACCG CGAGGCGGTT
4021 TTCTCCGGCG CGTAAAAATG CGCTCAGGTC AAATTCAGAC GGCAAACGAC TGTCCTGGCC
4081 GTAACCGACC CAGCGCCCGT TGCACCACAG ATGAAACGCC GAGTTAACGC CATCAAAAAT
4141 AATTCGCGTC TGGCCTTCCT GTAGCCAGCT TTCATCAACA TTAAATGTGA GCGAGTAACA
4201 ACCCGTCGGA TTCTCCGTGG GAACAAACGG CGGATTGACC GTAATGGGAT AGGTCACGTT
4261 GGTGTAGATG GGCGCATCGT AACCGTGCAT CTGCCAGTTT GAGGGGACGA CGACAGTATC
4321 GGCCTCAGGA AGATCGCACT CCAGCCAGCT TTCCGGCACC GCTTCTGGTG CCGGAAACCA
4381 GGCAAAGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
4441 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
4501 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GGATCTATCA TTTTTAGCAG
4561 TGATTCTAAT TGCAGCTGCT CTTTGATACA ACTAATTTTA CGACGACGAT GCGAGCTTTT
4621 ATTCAACCGA GCGTGCATGT TTGCAATCGT GCAAGCGTTA TCAATTTTTC ATTATCGTAT
4681 TGTTGCACAT CAACAGGCTG GACACCACGT TGAACTCGCC GCAGTTTTGC GGCAAGTTGG
4741 ACCCGCCGCG CATCCAATGC AAACTTTCCG ACATTCTGTT GCCTACGAAC GATTGATTCT
4801 TTGTCCATTG ATCGAAGCGA GTGCCTTCGA CTTTTTCGTG TCCAGTGTGG CTT