443143416YEplac195
- 產(chǎn)品貨號GS-1368
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 載體抗性Ampicillin (氨芐青霉素)
- 篩選標(biāo)記URA3
- 載體大小5241 bp
- 啟動子lac
One of 9 shuttle vectors (ATCC 87585 ? 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI?type) and yeast marker (LEU2, URA3 or TRP1). This is a YE?type (yeast episomal) vector with the URA3 marker. The PstI restriction site from the URA3 gene of S. cerevisiae was removed by using oligo?directed mutatgenesis.
0001 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
0061 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
0121 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
0181 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTTGC
0241 ATGCCTGCAG GTCGACTCTA GAGGATCCCC GGGTACCGAG CTCGAATTCA CTGGCCGTCG
0301 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC
0361 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC
0421 AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT
0481 GCGGTATTTC ACACCGCATA TATCGGATCG TACTTGTTAC CCATCATTGA ATTTTGAACA
0541 TCCGAACCTG GGAGTTTTCC CTGAAACAGA TAGTATATTT GAACCTGTAT AATAATATAT
0601 AGTCTAGCGC TTTACGGAAG ACAATGTATG TATTTCGGTT CCTGGAGAAA CTATTGCATC
0661 TATTGCATAG GTAATCTTGC ACGTCGCATC CCCGGTTCAT TTTCTGCGTT TCCATCTTGC
0721 ACTTCAATAG CATATCTTTG TTAACGAAGC ATCTGTGCTT CATTTTGTAG AACAAAAATG
0781 CAACGCGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA
0841 ATGCAACGCG AAAGCGCTAT TTTACCAACG AAGAATCTGT GCTTCATTTT TGTAAAACAA
0901 AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT TTTTACAGAA
0961 CAGAAATGCA ACGCGAGAGC GCTATTTTAC CAACAAAGAA TCTATACTTC TTTTTTGTTC
1021 TACAAAAATG CATCCCGAGA GCGCTATTTT TCTAACAAAG CATCTTAGAT TACTTTTTTT
1081 CTCCTTTGTG CGCTCTATAA TGCAGTCTCT TGATAACTTT TTGCACTGTA GGTCCGTTAA
1141 GGTTAGAAGA AGGCTACTTT GGTGTCTATT TTCTCTTCCA TAAAAAAAGC CTGACTCCAC
1201 TTCCCGCGTT TACTGATTAC TAGCGAAGCT GCGGGTGCAT TTTTTCAAGA TAAAGGCATC
1261 CCCGATTATA TTCTATACCG ATGTGGATTG CGCATACTTT GTGAACAGAA AGTGATAGCG
1321 TTGATGATTC TTCATTGGTC AGAAAATTAT GAACGGTTTC TTCTATTTTG TCTCTATATA
1381 CTACGTATAG GAAATGTTTA CATTTTCGTA TTGTTTTCGA TTCACTCTAT GAATAGTTCT
1441 TACTACAATT TTTTTGTCTA AAGAGTAATA CTAGAGATAA ACATAAAAAA TGTAGAGGTC
1501 GAGTTTAGAT GCAAGTTCAA GGAGCGAAAG GTGGATGGGT AGGTTATATA GGGATATAGC
1561 ACAGAGATAT ATAGCAAAGA GATACTTTTG AGCAATGTTT GTGGAAGCGG TATTCGCAAT
1621 ATTTTAGTAG CTCGTTACAG TCCGGTGCGT TTTTGGTTTT TTGAAAGTGC GTCTTCAGAG
1681 CGCTTTTGGT TTTCAAAAGC GCTCTGAAGT TCCTATACTT TCTAGCTAGA GAATAGGAAC
1741 TTCGGAATAG GAACTTCAAA GCGTTTCCGA AAACGAGCGC TTCCGAAAAT GCAACGCGAG
1801 CTGCGCACAT ACAGCTCACT GTTCACGTCG CACCTATATC TGCGTGTTGC CTGTATATAT
1861 ATATACATGA GAAGAACGGC ATAGTGCGTG TTTATGCTTA AATGCGTACT TATATGCGTC
1921 TATTTATGTA GGATGAAAGG TAGTCTAGTA CCTCCTGTGA TATTATCCCA TTCCATGCGG
1981 GGTATCGTAT GCTTCCTTCA GCACTACCCT TTAGCTGTTC TATATGCTGC CACTCCTCAA
2041 TTGGATTAGT CTCATCCTTC AATGCTATCA TTTCCTTTGA TATTGGATCG ATCCGATGAT
2101 AAGCTGTCAA ACATGAGAAT TGGGTAATAA CTGATATAAT TAAATTGAAG CTCTAATTTG
2161 TGAGTTTAGT ATACATGCAT TTACTTATAA TACAGTTTTT TAGTTTTGCT GGCCGCATCT
2221 TCTCAAATAT GCTTCCCAGC CTGCTTTTCT GTAACGTTCA CCCTCTACCT TAGCATCCCT
2281 TCCCTTTGCA AATAGTCCTC TTCCAACAAT AATAATGTCA GATCCTGTAG AGACCACATC
2341 ATCCACGGTT CTATACTGTT GACCCAATGC GTCTCCCTTG TCATCTAAAC CCACACCGGG
2401 TGTCATAATC AACCAATCGT AACCTTCATC TCTTCCACCC ATGTCTCTTT GAGCAATAAA
2461 GCCGATAACA AAATCTTTGT CGCTCTTCGC AATGTCAACA GTACCCTTAG TATATTCTCC
2521 AGTAGATAGG GAGCCCTTGC ATGACAATTC TGCTAACATC AAAAGGCCTC TAGGTTCCTT
2581 TGTTACTTCT TCTGCCGCCT GCTTCAAACC GCTAACAATA CCTGGGCCCA CCACACCGTG
2641 TGCATTCGTA ATGTCTGCCC ATTCTGCTAT TCTGTATACA CCCGCAGAGT ACTGCAATTT
2701 GACTGTATTA CCAATGTCAG CAAATTTTCT GTCTTCGAAG AGTAAAAAAT TGTACTTGGC
2761 GGATAATGCC TTTAGCGGCT TAACTGTGCC CTCCATGGAA AAATCAGTCA AGATATCCAC
2821 ATGTGTTTTT AGTAAACAAA TTTTGGGACC TAATGCTTCA ACTAACTCCA GTAATTCCTT
2881 GGTGGTACGA ACATCCAATG AAGCACACAA GTTTGTTTGC TTTTCGTGCA TGATATTAAA
2941 TAGCTTGGCA GCAACAGGAC TAGGATGAGT AGCAGCACGT TCCTTATATG TAGCTTTCGA
3001 CATGATTTAT CTTCGTTTCC TGCATGTTTT TGTTCTGTGC AGTTGGGTTA AGAATACTGG
3061 GCAATTTCAT GTTTCTTCAA CACTACATAT GCGTATATAT ACCAATCTAA GTCTGTGCTC
3121 CTTCCTTCGT TCTTCCTTCT GTTCGGAGAT TACCGAATCA AAAAAATTTC AAAGAAACCG
3181 AAATCAAAAA AAAGAATAAA AAAAAAATGA TGAATTGAAT TGAAAAGCTA ATTCTTGAAG
3241 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
3301 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
3361 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
3421 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
3481 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
3541 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
3601 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
3661 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
3721 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
3781 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
3841 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
3901 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
3961 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
4021 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT
4081 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
4141 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC
4201 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA
4261 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC
4321 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
4381 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
4441 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
4501 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
4561 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT
4621 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
4681 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
4741 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
4801 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
4861 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
4921 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
4981 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
5041 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
5101 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT
5161 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC
5221 AGTGAGCGAG GAAGCGGAAG A