443143416pYES2
- 產(chǎn)品貨號(hào)GS-1351
- 銷(xiāo)售價(jià)格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 表達(dá)類(lèi)型高
- 載體抗性Ampicillin (氨芐青霉素)
- 篩選標(biāo)記URA3
- 載體大小5857 bp
- 啟動(dòng)子GAL1
- 5'測(cè)序引物T7: TAATACGACTCACTATAGGG
- 備注說(shuō)明利用半乳糖誘導(dǎo)蛋白在釀酒酵母中表達(dá)
pYES2的是一個(gè)5.9 kb的載體，設(shè)計(jì)用來(lái)在釀酒酵母（Saccharomyces cerevisiae）中誘導(dǎo)表達(dá)重組蛋白。載體的特點(diǎn)在于基因插入載體的構(gòu)建簡(jiǎn)單，以及能夠使用原養(yǎng)型尿嘧啶進(jìn)行轉(zhuǎn)化株的篩選。 該載體包含以下元素：
- 酵母GAL1啟動(dòng)子，能夠在釀酒酵母中被半乳糖高水平的誘導(dǎo)蛋白表達(dá)目的蛋白，同時(shí)能夠被葡萄糖抑制表達(dá)
- 多克隆位點(diǎn)可以使用的很多限制酶切位點(diǎn)，便于基因插入。
- CYC1終止子能夠有效終止mRNA的轉(zhuǎn)錄。
- 能夠利用URA3基因篩選帶有ura3基因型的酵母宿主菌株轉(zhuǎn)化子。
- 氨芐抗性基因能夠方便在大腸桿菌中的進(jìn)行載體篩選。
0001 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
0061 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
0121 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
0181 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA
0241 CTAATTACAT GATGCGGCCC TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT
0301 CTGCAGAATT CCAGCACACT GGCGGCCGTT ACTAGTGGAT CCGAGCTCGG TACCAAGCTT
0361 AATATTCCCT ATAGTGAGTC GTATTACAGC TGCTAGTAGT CCGATCCGGG GTTTTTTCTC
0421 CTTGACGTTA AAGTATAGAG GTATATTAAC AATTTTTTGT TGATACTTTT ATTACATTTG
0481 AATAAGAAGT AATACAAACC GAAAATGTTG AAAGTATTAG TTAAAGTGGT TAATGCAGTT
0541 TTTGCATTTA TATATCTGTT AATAGATCAA AAATCATCGC TTCGCTGATT AATTACCCCA
0601 GAAATAAGGC TAAAAAACTA ATCGCATTAT CATCCTATGG TTGTTAATTT GATTCGTTCA
0661 TTTGAAGGTT TGTGGGGCCA GGTTACTGCC AATTTTTCCT CTTCATAACC ATAAAAGCTA
0721 GTATTGTAGA ATCTTTATTG TTCGGAGCAG TGCGGCGCGA GGCACATCTG CGTTTCAGGA
0781 ACGCGACCGG TGAGGACGAG GACGCACGGA GGAGAGTCTT CCTTCGGAGG GCTGTCACCC
0841 GCTCGGCGGC TTCTAATCCG TACTAGTGGA TCATCCCCAC GCGCCCTGTA GCGCCCCATT
0901 AAGCGCGGCG GGTGTGGTGG TTACGCCCAG CGTGACCCCT ACACTTCCCA CCGCCCTAGC
0961 CCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
1021 AGCTCTAAAT GGGGGCATCC GTTTACCCTT CCGATTTACT GCTTTACGGC ACCTCGACCC
1081 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACCCTTTT
1141 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTGG AAACTGGAAC
1201 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGG
1261 CTATTCGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
1321 AACGTTTACA ATTTAAATAT TTGCTTATAC AATCTTCCTG TTTTTGGGGC TTTTCTGATT
1381 ATCAACCGGG GTGGAGCTTC CCATTGCGAA TACCGCTTCC ACAAACATTG CTCAAAAGTA
1441 TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC CTACCCATCC ACCTTTCGCT
1501 CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT GTTTATCTCT AGTATTACTC
1561 TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG AATCGAAAAC AATACGAAAA
1621 TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG AAGAAACCGT TCATAATTTT
1681 CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC ACAAAGTATG CGCAATCCAC
1741 ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA AAATGCACCC GCAGCTTCGC
1801 TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT TATGGAAGAG AAAATAGACA
1861 CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC AAAAAGTTAT CAAGAGACTG
1921 CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA TGCTTTGTTA GAAAAATAGC
1981 GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA GATTCTTTGT TGGTAAAATA
2041 GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT GTTTGAAAAA
2101 TTAGCGCTCT CGTCGCGTTG CATTTTTGTT TTACAAAAAT GAAGCACAGA TTCTTCGTTG
2161 GTAAAATAGC GCTTTCGCGT TGCATTTCTG TTCTGTAAAA ATGCAGCTCA GATTCTTTGT
2221 TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTCTACA AAATGAAGCA CAGATGCTTC
2281 GTTAACAAAG ATATGCTATT GAAGTGCAAG ATGGAAACGC AGAAAATGAA CCGGGGATGC
2341 GACGTGCAAG ATTACCTATG CAATAGATGC AATAGTTTCT CCAGGAACCG AAATACATAC
2401 ATTGTCTTCC GTAAAGCGCT AGACTATATA TTATTATACA GGTTCAAATA TACTATCTGT
2461 TTCAGGGAAA ACTCCCAGGT TCGGATGTTC AAAATTCAAT GATGGGTAAC AAGTACGATC
2521 GTAAATCTGT AAAACAGTTT GTCGGATATT AGGCTGTATC TCCTCAAAGC GTATTCGAAT
2581 ATCATTGAGA AGCTGCAGCG TCACATCGGA TAATAATGAT GGCAGCCATT GTAGAAGTGC
2641 CTTTTGCATT TCTAGTCTCT TTCTCGGTCT AGCTAGTTTT ACTACATCGC GAAGATAGAA
2701 TCTTAGATCA CACTGCCTTT GCTGAGCTGG ATCAATAGAG TAACAAAAGA GTGGTAAGGC
2761 CTCGTTAAAG GACAAGGACC TGAGCGGAAG TGTATCGTAC AGTAGACGGA GTATCTAGTA
2821 TAGTCTATAG TCCGTGGAAT TAATTCTCAT CTTTGACAGC TTATCATCGA TAAGCTAGCT
2881 TTTCAATTCA ATTCATCATT TTTTTTTTAT TCTTTTTTTT GATTTCGGTT TCTTTGAAAT
2941 TTTTTTGATT CGGTAATCTC CGAACAGAAG GAAGAACGAA GGAAGGAGCA CAGACTTAGA
3001 TTGGTATATA TACGCATATG TAGTGTTGAA GAAACATGAA ATTGCCCAGT ATTCTTAACC
3061 CAACTGCACA GAACAAAAAC CTGCAGGAAA CGAAGATAAA TCATGTCGAA AGCTACATAT
3121 AAGGAACGTG CTGCTACTCA TCCTAGTCCT GTTGCTGCCA AGCTATTTAA TATCATGCAC
3181 GAAAAGCAAA CAAACTTGTG TGCTTCATTG GATGTTCGTA CCACCAAGGA ATTACTGGAG
3241 TTAGTTGAAG CATTAGGTCC CAAAATTTGT TTACTAAAAA CACATGTGGA TATCTTGACT
3301 GATTTTTCCA TGGAGGGCAC AGTTAAGCCG CTAAAGGCAT TATCCGCCAA GTACAATTTT
3361 TTACTCTTCG AAGACAGAAA ATTTGCTGAC ATTGGTAATA CAGTCAAATT GCAGTACTCT
3421 GCGGGTGTAT ACAGAATAGC AGAATGGGCA GACATTACGA ATGCACACGG TGTGGTGGGC
3481 CCAGGTATTG TTAGCGGTTT GAAGCAGGCG GCAGAAGAAG TAACAAAGGA ACCTAGAGGC
3541 CTTTTGATGT TAGCAGAATT GTCATGCAAG GGCTCCCTAT CTACTGGAGA ATATACTAAG
3601 GGTACTGTTG ACATTGCGAA GAGCGACAAA GATTTTGTTA TCGGCTTTAT TGCTCAAAGA
3661 GACATGGGTG GAAGAGATGA AGGTTACGAT TGGTTGATTA TGACACCCGG TGTGGGTTTA
3721 GATGACAAGG GAGACGCATT GGGTCAACAG TATAGAACCG TGGATGATGT GGTCTCTACA
3781 GGATCTGACA TTATTATTGT TGGAAGAGGA CTATTTGCAA AGGGAAGGGA TGCTAAGGTA
3841 GAGGGTGAAC GTTACAGAAA AGCAGGCTGG GAAGCATATT TGAGAAGATG CGGCCAGCAA
3901 AACTAAAAAA CTGTATTATA AGTAAATGCA TGTATACTAA ACTCACAAAT TAGAGCTTCA
3961 ATTTAATTAT ATCAGTTATT ACCCATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
4021 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
4081 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
4141 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
4201 TGAGCACTTT TAAAGTTCTG CTATGTGATA CACTATTATC CCGTATTGAC GCCGGGCAAG
4261 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
4321 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
4381 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
4441 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
4501 TGAATGAAGC CATACCAAAC GACGAGAGTG ACACCACGAT GCCTGTAGCA ATGCCAACAA
4561 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
4621 ACTGAATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
4681 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
4741 TGGGGCCAGA TGGTAAGCGC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
4801 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
4861 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
4921 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
4981 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
5041 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
5101 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
5161 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
5221 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
5281 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
5341 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
5401 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
5461 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
5521 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
5581 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
5641 TTTTACGGTT CCTGGGCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
5701 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
5761 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC
5821 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAG