443143416pVitro2-neo-mcs
- 產(chǎn)品貨號GS-1043
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 表達類型高
- 載體抗性Kanamycin (卡那霉素)
- 篩選標記Neo
- 載體大小6125 bp
- 啟動子CMV
- 5'測序引物EF-1a-F：TCAAGCCTCAGACAGTGGTTC
Expression plasmids pVITRO2-MCS are developed mainly for?in vitro?studies.?
pVITRO2-MCS allows the ubiquitous and constitutive?co-expression of two genes of interest.
pVITRO2-MCS plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-MCS plasmids are available with different selectable markers that are active both in?E. coli?and mammalian cells.
pVITRO2-MCS plasmids contain two multiple cloning sites (MCS) for the convenient cloning of your cDNAs of interest.
0001 CCTGCAGGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
0061 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT
0121 TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
0181 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT
0241 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC
0301 GCTATTACCA TGATGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
0361 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGACTAGTC
0421 AGGGCCCCAA CCCCCCCAAG CCCCCATTTC ACAACACGCT GGCGCTACAG GCGCGTGACT
0481 TCCCCTTGCT TTGGGGCGGG GGGCTGAGAC TCCTATGTGC TCCGGATTGG TCAGGCACGG
0541 CCTTCGGCCC CGCCTCCTGC CACCGCAGAT TGGCCGCTAG GCCTCCCCGA GCGCCCTGCC
0601 TCCGAGGGCC GGCGCACCAT AAAAGAAGCC GCCCTAGCCA CGTCCCCTCG CAGTTCGGCG
0661 GTCCCGCGGG TCTGTCTCAA GCTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC
0721 CGCGGGCCTG GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCATGCCC
0781 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC
0841 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCTTGGGCG
0901 CTGGGGCCGC CGCGTGCTAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGCTAA
0961 GTCTCTAGCC ATTTAAAATT TTTGATAACC AGCTGCGACG CTTTTTTTCT GGCGAGATAG
1021 TCTTGTAAAT GCGGGCCAGG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG
1081 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC
1141 ACCGAGAATC GGACGGGGGT AGTCTCAAAC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC
1201 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG
1261 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG
1321 CCCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CTTCCTCATC
1381 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTGTC
1441 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC
1501 CCACACTGAG TGGGTGGAGA CTGAAGAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC
1561 TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGCCTCATTC TCAAGCCTCA GACAGTGGTT
1621 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAAAACTAC CCCTAAAAGC CACCGGCGTG
1681 CGCAAGATCT GAATTCTTCG AACTCGAGGC TAGCTGGCCA GACATGATAA GATACATTGA
1741 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
1801 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
1861 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
1921 AAACCTCTAC AAATGTGGTA TGGAAATGTT AATTAACTAG CCATGACCAA AATCCCTTAA
1981 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
2041 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
2101 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
2161 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
2221 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
2281 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
2341 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
2401 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
2461 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
2521 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
2581 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
2641 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT AATTAACCTG
2701 CAGGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGG TACCTTCTGA GGCTGAAAGA
2761 ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA
2821 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT
2881 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCAC
2941 TAGTTCCGCC AGAGCGCGCG AGGGCCTCCA GCGGCCGCCC CTCCCCCACA GCAGGGGCGG
3001 GGTCCCGCGC CCACCGGAAG GAGCGGGCTC GGGGCGGGCG GCGCTGATTG GCCGGGGCGG
3061 GCCTGACGCC GACGCGGCTA TAAGAGACCA CAAGCGACCC GCAGGGCCAG ACGTTCTTCG
3121 CCGAAGCTTG CCGTCAGAAC GCAGGTGAGG GGCGGGTGTG GCTTCCGCGG GCCGCCGAGC
3181 TGGAGGTCCT GCTCCGAGCG GGCCGGGCCC CGCTGTCGTC GGCGGGGATT AGCTGCGAGC
3241 ATTCCCGCTT CGAGTTGCGG GCGGCGCGGG AGGCAGAGTG CGAGGCCTAG CGGCAACCCC
3301 GTAGCCTCGC CTCGTGTCCG GCTTGAGGCC TAGCGTGGTG TCCGCGCCGC CGCCGCGTGC
3361 TACTCCGGCC GCACTCTGGT CTTTTTTTTT TTTGTTGTTG TTGCCCTGCT GCCTTCGATT
3421 GCCGTTCAGC AATAGGGGCT AACAAAGGGA GGGTGCGGGG CTTGCTCGCC CGGAGCCCGG
3481 AGAGGTCATG GTTGGGGAGG AATGGAGGGA CAGGAGTGGC GGCTGGGGCC CGCCCGCCTT
3541 CGGAGCACAT GTCCGACGCC ACCTGGATGG GGCGAGGCCT GGGGTTTTTC CCGAAGCAAC
3601 CAGGCTGGGG TTAGCGTGCC GAGGCCATGT GGCCCCAGCA CCCGGCACGA TCTGGCTTGG
3661 CGGCGCCGCG TTGCCCTGCC TCCCTAACTA GGGTGAGGCC ATCCCGTCCG GCACCAGTTG
3721 CGTGCGTGGA AAGATGGCCG CTCCCGGGCC CTGTTGCAAG GAGCTCAAAA TGGAGGACGC
3781 GGCAGCCCGG TGGAGCGGGC GGGTGAGTCA CCCACACAAA GGAAGAGGGC CTGGTCCCTC
3841 ACCGGCTGCT GCTTCCTGTG ACCCCGTGGT CCTATCGGCC GCAATAGTCA CCTCGGGCTT
3901 TTGAGCACGG CTAGTCGCGG CGGGGGGAGG GGATGTAATG GCGTTGGAGT TTGTTCACAT
3961 TTGGTGGGTG GAGACTAGTC AGGCCAGCCT GGCGCTGGAA GTCATTTTTG GAATTTGTCC
4021 CCTTGAGTTT TGAGCGGAGC TAATTCTCGG GCTTCTTAGC GGTTCAAAGG TATCTTTTAA
4081 ACCCTTTTTT AGGTGTTGTG AAAACCACCG CTAATTCAAA GCAACCGGTG ATATCGGATC
4141 CACGCGTATC GATTGTCGAC CCTAGGAGCA GGTTTCCCCA ATGACACAAA ACGTGCAACT
4201 TGAAACTCCG CCTGGTCTTT CCAGGTCTAG AGGGGTAACA CTTTGTACTG CGTTTGGCTC
4261 CACGCTCGAT CCACTGGCGA GTGTTAGTAA CAGCACTGTT GCTTCGTAGC GGAGCATGAC
4321 GGCCGTGGGA ACTCCTCCTT GGTAACAAGG ACCCACGGGG CCAAAAGCCA CGCCCACACG
4381 GGCCCGTCAT GTGTGCAACC CCAGCACGGC GACTTTACTG CGAAACCCAC TTTAAAGTGA
4441 CATTGAAACT GGTACCCACA CACTGGTGAC AGGCTAAGGA TGCCCTTCAG GTACCCCGAG
4501 GTAACACGCG ACACTCGGGA TCTGAGAAGG GGACTGGGGC TTCTATAAAA GCGCTCGGTT
4561 TAAAAAGCTT CTATGCCTGA ATAGGTGACC GGAGGTCGGC ACCTTTCCTT TGCAATTACT
4621 GACCCTATGA ATACAACTGA CTGTTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG
4681 TATAATACGA CTCACTATAG GAGGGCCACC ATGATTGAAC AAGATGGATT GCACGCAGGT
4741 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
4801 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
4861 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG
4921 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
4981 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
5041 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
5101 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
5161 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
5221 TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC TCGTCGTGAC ACATGGCGAT
5281 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
5341 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
5401 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
5461 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT
5521 TCGAAATGAC CGACCAAGCG AATTCGCTAG GATTATCCCT AATACCTGCC ACCCCACTCT
5581 TAATCAGTGG TGGAAGAACG GTCTCAGAAC TGTTTGTTTC AATTGGCCAT TTAAGTTTAG
5641 TAGTAAAAGA CTGGTTAATG ATAACAATGC ATCGTAAAAC CTTCAGAAGG AAAGGAGAAT
5701 GTTTTGTGGA CCACTTTGGT TTTCTTTTTT GCGTGTGGCA GTTTTAAGTT ATTAGTTTTT
5761 AAAATCAGTA CTTTTTAATG GAAACAACTT GACCAAAAAT TTGTCACAGA ATTTTGAGAC
5821 CCATTAAAAA AGTTAAATGA GAAACCTGTG TGTTCCTTTG GTCAACACCG AGACATTTAG
5881 GTGAAAGACA TCTAATTCTG GTTTTACGAA TCTGGAAACT TCTTGAAAAT GTAATTCTTG
5941 AGTTAACACT TCTGGGTGGA GAATAGGGTT GTTTTCCCCC CACATAATTG GAAGGGGAAG
6001 GAATATCATT TAAAGCTATG GGAGGGTTGC TTTGATTACA ACACTGGAGA GAAATGCAGC
6061 ATGTTGCTGA TTGCCTGTCA CTAAAACAGG CCAAAAACTG AGTCCTTGGG TTGCATAGAA
6121 AGCTG