443143416pTrc-CKS
- 產(chǎn)品貨號GS-2235
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 載體抗性Ampicillin
- 啟動子Trc
- 5'測序引物5 ATC CAT GTT GCT GTT GCT CAG G 3
pTrc-CKS表達(dá)載體采用E.coliTrc啟動子和蛋白質(zhì)工程改造過CKS (CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase or CMP-KDO synthetase)基因融合表達(dá)，同時選用人鼻病毒14亞型3C蛋白酶專一性切割位點。CKS融合表達(dá)是美國Abbot公司的專利，CKS融合蛋白研制成功HIV和HCV重組抗原并取得FDA認(rèn)證在美國上市。CKS是一個非常穩(wěn)定且高度可溶的理想融合伴侶。本發(fā)明除去掉CKS C端8個氨基酸外，還將C端Met突變成Thr
CKSmut(E.coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase or CMP-KDO synthetase)編碼氨基酸序列如下(含多克隆位點)：
MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAERIIVATDHEDVARAVEAAGGEVCMTRADHQSGTE
RLAEVVEKCAFSDDTVIVNVQGDEPMIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSR
ATIPWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIETLEQLRVLWYGEKIHVAVAQEVPGTGVDTP
EDGSMPDSLEVLFQGPAGSAEFSASHHHHHH
268 Amino Acids??? ?Molecular Weight 29KD
