pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable cell lines using the Tet-On? or Tet-Off? Gene Expression Systems because the absence of an enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived plasmids upon cointegration into the host cell's genome.

載體應(yīng)用

pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains a gene of interest using any standard transfection technique. Most mammalian cell lines require 200 μg/ml of Hygromycin to select for stably transformed cells.

0001 CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCCAGACATG ATAAGATACA TTGATGAGTT 
0061 TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC 
0121 TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT 
0181 TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT 
0241 CTACAAATGT GGTATGGCTG ATTATGATCC CCAGGAAGCT CCTCTGTGTC CTCATAAACC 
0301 CTAACCTCCT CTACTTGAGA GGACATTCCA ATCATAGGCT GCCCATCCAC CCTCTGTGTC 
0361 CTCCTGTTAA TTAGGTCACT TAACAAAAAG GAAATTGGGT AGGGGTTTTT CACAGACCGC 
0421 TTTCTAAGGG TAATTTTAAA ATATCTGGGA AGTCCCTTCC ACTGCTGTGT TCCAGAAGTG 
0481 TTGGTAAACA GCCCACAAAT GTCAACAGCA GAAACATACA AGCTGTCAGC TTTGCACAAG 
0541 GGCCCAACAC CCTGCTCATC AAGAAGCACT GTGGTTGCTG TGTTAGTAAT GTGCAAAACA 
0601 GGAGGCACAT TTTCCCCACC TGTGTAGGTT CCAAAATATC TAGTGTTTTC ATTTTTACTT 
0661 GGATCAGGAA CCCAGCACTC CACTGGATAA GCATTATCCT TATCCAAAAC AGCCTTGTGG 
0721 TCAGTGTTCA TCTGCTGACT GTCAACTGTA GCATTTTTTG GGGTTACAGT TTGAGCAGGA 
0781 TATTTGGTCC TGTAGTTTGC TAACACACCC TGCAGCTCCA AAGGTTCCCC ACCAACAGCA 
0841 AAAAAATGAA AATTTGACCC TTGAATGGGT TTTCCAGCAC CATTTTCATG AGTTTTTTGT 
0901 GTCCCTGAAT GCAAGTTTAA CATAGCAGTT ACCCCAATAA CCTCAGTTTT AACAGTAACA 
0961 GCTTCCCACA TCAAAATATT TCCACAGGTT AAGTCCTCAT TTAAATTAGG CAAAGGAATT 
1021 CCTCGACCTG CAGCCCAAGC TTGGCACTGG CGCCAGAAAT CCGCGCGGTG GTTTTTGGGG 
1081 GTCGGGGGTG TTTGGCAGCC ACAGACGCCC GGTGTTCGTG TCGCGCCAGT ACATGCGGTC 
1141 CATGCCCAGG CCATCCAAAA ACCATGGGTC TGTCTGCTCA GTCCAGTCGT GGACCTGACC 
1201 CCACGCAACG CCCAAAAGAA TAACCCCCAC GAACCATAAA CCATTCCCCA TGGGGGACCC 
1261 CGTCCCTAAC CCACGGGGCC CGTGGCTATG GCGGGCTTGC CGCCCCGACG TTGGCTGCGA 
1321 GCCCTGGGCC TTCACCCGAA CTTGGGGGTT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT 
1381 ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC 
1441 CGCGTTTATG AACAAACGAC CCAACACCCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC 
1501 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCATCTC 
1561 CCGATCCCCA CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC 
1621 ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG TCCCGGCTCC 
1681 GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT GCATCATCGA AATTGCCGTC 
1741 AACCAAGCTC TGATAGAGTT GGTCAAGACC AATGCGGAGC ATATACGCCC GGAGCCGCGG 
1801 CGATCCTGCA AGCTCCGGAT GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC 
1861 CAACCACGGC CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC 
1921 CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT TGTTGGAGCC 
1981 GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC TCGGCCCAAA GCATCAGCTC 
2041 ATCGAGAGCC TGCGCGACGG ACGCACTGAC GGTGTCGTCC ATCACAGTTT GCCAGTGATA 
2101 CACATGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC 
2161 TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC 
2221 CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA 
2281 CGTGACACCC TGTGCACGGC GGGAGATGCA ATAGGTCAGG CTCTCGCTGA ATTCCCCAAT 
2341 GTCAAGCACT TCCGGAATCG GGAGCGCGGC CGATGCAAAG TGCCGATAAA CATAACGATC 
2401 TTTGTAGAAA CCATCGGCGC AGCTATTTAC CCGCAGGACA TATCCACGCC CTCCTACATC 
2461 GAAGCTGAAA GCACGAGATT CTTCGCCCTC CGAGAGCTGC ATCAGGTCGG AGACGCTGTC 
2521 GAACTTTTCG ATCAGAAACT TCTCGACAGA CGTCGCGGTG AGTTCAGGCT TTTTCATATC 
2581 AAGCTGATCT TGCGGCACGC TGTTGACGCT GTTAAGCGGG TCGCTGCAGG GTCGCTCGGT 
2641 GTTCGAGGCC ACACGCGTCA CCTTAATATG CGAAGTGGAC CTGGGACCGC GCCGCCCCGA 
2701 CTGCATCTGC GTGTTCGAAT TCGCCAATGA CAAGACGCTG GGCGGGGTTT GTGTCATCAT 
2761 AGAACTAAAG ACATGCAAAT ATATTTCTTC CGGGGACACC GCCAGCAAAC GCGAGCAACG 
2821 GGCCACGGGG ATGAAGCAGC CCGGCGGCAC CTCGCTAACG GATTCACCAC TCCAAGAATT 
2881 GGAGCCAATC AATTCTTGCG GAGAACTGTG AATGCGCAAA CCAACCCTTG GCAGAACATA 
2941 TCCATCGCGT CCGCCATCTC CAGCAGCCGC ACGCGGCGCA TCTCGGGGCC GACGCGCTGG 
3001 GCTACGTCTT GCTGGCGTTC GCGACGCGAG GCTGGATGGC CTTCCCCATT ATGATTCTTC 
3061 TCGCTTCCGG CGGCATCGGG ATGCCCGCGT TGCAGGCCAT GCTGTCCAGG CAGGTAGATG 
3121 ACGACCATCA GGGACAGCTT CAAGGATCGC TCGCGGCTCT TACCAGCGCC AGCAAAAGGC 
3181 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
3241 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
3301 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
3361 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
3421 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
3481 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
3541 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
3601 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 
3661 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
3721 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
3781 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
3841 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
3901 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 
3961 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 
4021 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 
4081 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 
4141 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 
4201 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 
4261 TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 
4321 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 
4381 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 
4441 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 
4501 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 
4561 GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 
4621 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 
4681 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 
4741 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 
4801 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 
4861 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 
4921 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 
4981 TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCAAGA 
5041 ATTCTCATGT TTGACAGCTT ATCATC